Cross-species testing of 27 pre-existing microsatellites in Podarcis gaigeae and Podarcis hispanica (Squamata: Lacertidae)

Anna Runemark; Marianne Gabirot; Staffan Bensch; Erik Svensson; Jose Martin; Panayiotis Pafilis; Efstratios D. Valakos; Bengt Hansson

doi:10.1111/j.1755-0998.2008.02299.x

Cross-species testing of 27 pre-existing microsatellites in Podarcis gaigeae and Podarcis hispanica (Squamata: Lacertidae)

Anna Runemark, Marianne Gabirot, Staffan Bensch, Erik Svensson, Jose Martin, Panayiotis Pafilis, Efstratios D. Valakos, Bengt Hansson

Research output: Contribution to journal › Article › peer-review

Abstract

We tested 27 microsatellite loci for cross-species amplification in the lacertids Podarcis gaigeae and Podarcis hispanica. We detected 11 and 15 polymorphic loci in the former and the latter species, respectively. In a larger sample of individuals from a single population of each species, the number of alleles ranged from five to 23 in 10 of the polymorphic loci in P. gaigeae, and between four and 13 in nine of polymorphic loci in P. hispanica. Two locus deviated from Hardy-Weinberg equilibrium in P. hispanica. Between 11 and 16 of the 27 loci also amplified successfully in three other Podarcis species.

Original language	English
Pages (from-to)	1367-1370
Journal	Molecular Ecology Resources
Volume	8
Issue number	6
DOIs	https://doi.org/10.1111/j.1755-0998.2008.02299.x
Publication status	Published - 2008

Subject classification (UKÄ)

Biological Sciences

Free keywords

neutral molecular marker
cross-species amplification
microsatellite
Podarcis
population differentiation
Squamata

Access to Document

10.1111/j.1755-0998.2008.02299.x

Cite this

@article{8c249a580290436a8490182379345d2f,

title = "Cross-species testing of 27 pre-existing microsatellites in Podarcis gaigeae and Podarcis hispanica (Squamata: Lacertidae)",

abstract = "We tested 27 microsatellite loci for cross-species amplification in the lacertids Podarcis gaigeae and Podarcis hispanica. We detected 11 and 15 polymorphic loci in the former and the latter species, respectively. In a larger sample of individuals from a single population of each species, the number of alleles ranged from five to 23 in 10 of the polymorphic loci in P. gaigeae, and between four and 13 in nine of polymorphic loci in P. hispanica. Two locus deviated from Hardy-Weinberg equilibrium in P. hispanica. Between 11 and 16 of the 27 loci also amplified successfully in three other Podarcis species.",

keywords = "neutral molecular marker, cross-species amplification, microsatellite, Podarcis, population differentiation, Squamata",

author = "Anna Runemark and Marianne Gabirot and Staffan Bensch and Erik Svensson and Jose Martin and Panayiotis Pafilis and Valakos, {Efstratios D.} and Bengt Hansson",

year = "2008",

doi = "10.1111/j.1755-0998.2008.02299.x",

language = "English",

volume = "8",

pages = "1367--1370",

journal = "Molecular Ecology Resources",

issn = "1755-098X",

publisher = "Wiley-Blackwell",

number = "6",

}

TY - JOUR

T1 - Cross-species testing of 27 pre-existing microsatellites in Podarcis gaigeae and Podarcis hispanica (Squamata: Lacertidae)

AU - Runemark, Anna

AU - Gabirot, Marianne

AU - Bensch, Staffan

AU - Svensson, Erik

AU - Martin, Jose

AU - Pafilis, Panayiotis

AU - Valakos, Efstratios D.

AU - Hansson, Bengt

PY - 2008

Y1 - 2008

N2 - We tested 27 microsatellite loci for cross-species amplification in the lacertids Podarcis gaigeae and Podarcis hispanica. We detected 11 and 15 polymorphic loci in the former and the latter species, respectively. In a larger sample of individuals from a single population of each species, the number of alleles ranged from five to 23 in 10 of the polymorphic loci in P. gaigeae, and between four and 13 in nine of polymorphic loci in P. hispanica. Two locus deviated from Hardy-Weinberg equilibrium in P. hispanica. Between 11 and 16 of the 27 loci also amplified successfully in three other Podarcis species.

AB - We tested 27 microsatellite loci for cross-species amplification in the lacertids Podarcis gaigeae and Podarcis hispanica. We detected 11 and 15 polymorphic loci in the former and the latter species, respectively. In a larger sample of individuals from a single population of each species, the number of alleles ranged from five to 23 in 10 of the polymorphic loci in P. gaigeae, and between four and 13 in nine of polymorphic loci in P. hispanica. Two locus deviated from Hardy-Weinberg equilibrium in P. hispanica. Between 11 and 16 of the 27 loci also amplified successfully in three other Podarcis species.

KW - neutral molecular marker

KW - cross-species amplification

KW - microsatellite

KW - Podarcis

KW - population differentiation

KW - Squamata

U2 - 10.1111/j.1755-0998.2008.02299.x

DO - 10.1111/j.1755-0998.2008.02299.x

M3 - Article

SN - 1755-098X

VL - 8

SP - 1367

EP - 1370

JO - Molecular Ecology Resources

JF - Molecular Ecology Resources

IS - 6

ER -

Cross-species testing of 27 pre-existing microsatellites in Podarcis gaigeae and Podarcis hispanica (Squamata: Lacertidae)

Abstract

Subject classification (UKÄ)

Free keywords

Access to Document

Fingerprint

Cite this