Cryo-EM Reveals Promoter DNA Binding and Conformational Flexibility of the General Transcription Factor TFIID

Hans Elmlund, Vera Baraznenok, Tomas Linder, Zsolt Szilagyi, Reza Rofougaran, Anders Hofer, Hans Hebert, Martin Lindahl, Claes M. Gustafsson

Research output: Contribution to journalArticlepeer-review

27 Citations (SciVal)

Abstract

The general transcription factor IID (TFIID) is required for initiation of RNA polymerase II-dependent transcription at many eukaryotic promoters. TFIID comprises the TATA-binding protein (TBP) and several conserved TBP-associated factors (TAFs). Recognition of the core promoter by TFIID assists assembly of the preinitiation complex. Using cryo-electron microscopy in combination with methods for ab initio single-particle reconstruction and heterogeneity analysis, we have produced density maps of two conformational states of Schizosaccharomyces pombe TFIID, containing and lacking TBP. We report that TBP-binding is coupled to a massive histone-fold domain rearrangement. Moreover, docking of the TBP-TAF1(N-terminus) atomic structure to the THID map and reconstruction of a TAF-promoter DNA complex helps to account for TAF-dependent regulation of promoter-TBP and promoter-TAF interactions.
Original languageEnglish
Pages (from-to)1442-1452
JournalStructure
Volume17
Issue number11
DOIs
Publication statusPublished - 2009

Subject classification (UKÄ)

  • Biological Sciences

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