Cryogels with affinity ligands as tools in protein purification

Solmaz Hajizadeh, Bo Mattiasson

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review

Abstract

Affinity chromatography is one of the well-known separation techniques especially if high purity is desired. Introducing ligands on monolithic structure gives the possibility for purifying complex media such as plasma and crude extract. This chapter is focusing on the preparation of cryogels as monolithic column and immobilization of concanavalin A on its surface as ligand for capturing the glycoprotein horseradish peroxidase.
Original languageEnglish
Title of host publicationAffinity Chromatography
EditorsSenta Reichelt
Place of PublicationNew York, NY
PublisherHumana Press
Pages183
Number of pages200
ISBN (Electronic)978-1-4939-2447-9
ISBN (Print)978-1-4939-2446-2
DOIs
Publication statusPublished - 2015

Publication series

NameMethods in Molecular Biology
PublisherHumana Press, New York, NY
ISSN (Electronic)1940-6029

Subject classification (UKÄ)

  • Biochemistry and Molecular Biology

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