Cryogels with affinity ligands as tools in protein purification

Solmaz Hajizadeh, Bo Mattiasson

    Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review

    Abstract

    Affinity chromatography is one of the well-known separation techniques especially if high purity is desired. Introducing ligands on monolithic structure gives the possibility for purifying complex media such as plasma and crude extract. This chapter is focusing on the preparation of cryogels as monolithic column and immobilization of concanavalin A on its surface as ligand for capturing the glycoprotein horseradish peroxidase.
    Original languageEnglish
    Title of host publicationAffinity Chromatography
    EditorsSenta Reichelt
    Place of PublicationNew York, NY
    PublisherHumana Press
    Pages183
    Number of pages200
    ISBN (Electronic)978-1-4939-2447-9
    ISBN (Print)978-1-4939-2446-2
    DOIs
    Publication statusPublished - 2015

    Publication series

    NameMethods in Molecular Biology
    PublisherHumana Press, New York, NY
    ISSN (Electronic)1940-6029

    Subject classification (UKÄ)

    • Biological Sciences

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