Crystallization and preliminary X-ray analysis of human dUTPase

G Larsson; Per-Olof Nyman; L A Svensson

doi:10.1107/S0907444996003277

Crystallization and preliminary X-ray analysis of human dUTPase

G Larsson, Per-Olof Nyman, L A Svensson

Biochemistry and Structural Biology

Research output: Contribution to journal › Article › peer-review

Abstract

Human dUTPase, expressed in Escherichia coli, has been crystallized. Single crystals were obtained by the vapour-diffusion technique using 2-propanol and PEG 4000 as precipitants. The enzyme was co-crystallized with the substrate dUTP and a metal chelator EDTA to prevent hydrolysis of the substrate. The crystals belong to the orthorombic space group P212121 with cell dimensions a = 67.51, b = 68.26 and c = 91.00 Å. The crystallographic asymmetric unit contains one trimer of identical subunits.

Original language	English
Pages (from-to)	1039-1040
Journal	Acta Crystallographica. Section D: Biological Crystallography
Volume	D52
Issue number	5
DOIs	https://doi.org/10.1107/S0907444996003277
Publication status	Published - 1996

Subject classification (UKÄ)

Structural Biology

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10.1107/S0907444996003277

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title = "Crystallization and preliminary X-ray analysis of human dUTPase",

abstract = "Human dUTPase, expressed in Escherichia coli, has been crystallized. Single crystals were obtained by the vapour-diffusion technique using 2-propanol and PEG 4000 as precipitants. The enzyme was co-crystallized with the substrate dUTP and a metal chelator EDTA to prevent hydrolysis of the substrate. The crystals belong to the orthorombic space group P212121 with cell dimensions a = 67.51, b = 68.26 and c = 91.00 {\AA}. The crystallographic asymmetric unit contains one trimer of identical subunits.",

author = "G Larsson and Per-Olof Nyman and Svensson, {L A}",

year = "1996",

doi = "10.1107/S0907444996003277",

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volume = "D52",

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journal = "Acta Crystallographica. Section D: Biological Crystallography",

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T1 - Crystallization and preliminary X-ray analysis of human dUTPase

AU - Larsson, G

AU - Nyman, Per-Olof

AU - Svensson, L A

PY - 1996

Y1 - 1996

N2 - Human dUTPase, expressed in Escherichia coli, has been crystallized. Single crystals were obtained by the vapour-diffusion technique using 2-propanol and PEG 4000 as precipitants. The enzyme was co-crystallized with the substrate dUTP and a metal chelator EDTA to prevent hydrolysis of the substrate. The crystals belong to the orthorombic space group P212121 with cell dimensions a = 67.51, b = 68.26 and c = 91.00 Å. The crystallographic asymmetric unit contains one trimer of identical subunits.

AB - Human dUTPase, expressed in Escherichia coli, has been crystallized. Single crystals were obtained by the vapour-diffusion technique using 2-propanol and PEG 4000 as precipitants. The enzyme was co-crystallized with the substrate dUTP and a metal chelator EDTA to prevent hydrolysis of the substrate. The crystals belong to the orthorombic space group P212121 with cell dimensions a = 67.51, b = 68.26 and c = 91.00 Å. The crystallographic asymmetric unit contains one trimer of identical subunits.

U2 - 10.1107/S0907444996003277

DO - 10.1107/S0907444996003277

M3 - Article

SN - 1399-0047

VL - D52

SP - 1039

EP - 1040

JO - Acta Crystallographica. Section D: Biological Crystallography

JF - Acta Crystallographica. Section D: Biological Crystallography

IS - 5

ER -

Crystallization and preliminary X-ray analysis of human dUTPase

Abstract

Subject classification (UKÄ)

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