Crystallization and preliminary X-ray analysis of the Rhodobacter capsulatus magnesium chelatase BchI subunit

R D Willows; Mats Hansson; S I Beale; Martin Laurberg; Salam Al-Karadaghi

doi:10.1107/S0907444998014759

Crystallization and preliminary X-ray analysis of the Rhodobacter capsulatus magnesium chelatase BchI subunit

R D Willows, Mats Hansson, S I Beale, Martin Laurberg, Salam Al-Karadaghi

Biochemistry and Structural Biology

Research output: Contribution to journal › Article › peer-review

Abstract

The Rhodobacter capsulatus BchI protein is one of three subunits of Mg chelatase, the enzyme which catalyzes the first committed step of chlorophyll and bacteriochlorophyll biosynthesis. The BchI protein was produced with an inducible T7 RNA polymerase expression system in Escherichia coli. The protein was purified from the soluble cell-extract fraction and crystallized from polyethylene glycol solution. The crystals diffract to a minimum Bragg spacing of 2.1 Å. The space group is P63 with unit-cell dimensions a = b = 90.6, c = 84.1 Å.

Original language	English
Pages (from-to)	689-690
Journal	Acta Crystallographica. Section D: Biological Crystallography
Volume	D55
DOIs	https://doi.org/10.1107/S0907444998014759
Publication status	Published - 1999

Subject classification (UKÄ)

Structural Biology

Free keywords

bacteriochlorophyll.
chlorophyll
magnesium chelatase BchI subunit

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10.1107/S0907444998014759

Cite this

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title = "Crystallization and preliminary X-ray analysis of the Rhodobacter capsulatus magnesium chelatase BchI subunit",

abstract = "The Rhodobacter capsulatus BchI protein is one of three subunits of Mg chelatase, the enzyme which catalyzes the first committed step of chlorophyll and bacteriochlorophyll biosynthesis. The BchI protein was produced with an inducible T7 RNA polymerase expression system in Escherichia coli. The protein was purified from the soluble cell-extract fraction and crystallized from polyethylene glycol solution. The crystals diffract to a minimum Bragg spacing of 2.1 {\AA}. The space group is P63 with unit-cell dimensions a = b = 90.6, c = 84.1 {\AA}.",

keywords = "bacteriochlorophyll., chlorophyll, magnesium chelatase BchI subunit",

author = "Willows, {R D} and Mats Hansson and Beale, {S I} and Martin Laurberg and Salam Al-Karadaghi",

year = "1999",

doi = "10.1107/S0907444998014759",

language = "English",

volume = "D55",

pages = "689--690",

journal = "Acta Crystallographica. Section D: Biological Crystallography",

issn = "1399-0047",

publisher = "John Wiley & Sons Inc.",

}

TY - JOUR

T1 - Crystallization and preliminary X-ray analysis of the Rhodobacter capsulatus magnesium chelatase BchI subunit

AU - Willows, R D

AU - Hansson, Mats

AU - Beale, S I

AU - Laurberg, Martin

AU - Al-Karadaghi, Salam

PY - 1999

Y1 - 1999

N2 - The Rhodobacter capsulatus BchI protein is one of three subunits of Mg chelatase, the enzyme which catalyzes the first committed step of chlorophyll and bacteriochlorophyll biosynthesis. The BchI protein was produced with an inducible T7 RNA polymerase expression system in Escherichia coli. The protein was purified from the soluble cell-extract fraction and crystallized from polyethylene glycol solution. The crystals diffract to a minimum Bragg spacing of 2.1 Å. The space group is P63 with unit-cell dimensions a = b = 90.6, c = 84.1 Å.

AB - The Rhodobacter capsulatus BchI protein is one of three subunits of Mg chelatase, the enzyme which catalyzes the first committed step of chlorophyll and bacteriochlorophyll biosynthesis. The BchI protein was produced with an inducible T7 RNA polymerase expression system in Escherichia coli. The protein was purified from the soluble cell-extract fraction and crystallized from polyethylene glycol solution. The crystals diffract to a minimum Bragg spacing of 2.1 Å. The space group is P63 with unit-cell dimensions a = b = 90.6, c = 84.1 Å.

KW - bacteriochlorophyll.

KW - chlorophyll

KW - magnesium chelatase BchI subunit

U2 - 10.1107/S0907444998014759

DO - 10.1107/S0907444998014759

M3 - Article

SN - 1399-0047

VL - D55

SP - 689

EP - 690

JO - Acta Crystallographica. Section D: Biological Crystallography

JF - Acta Crystallographica. Section D: Biological Crystallography

ER -

Crystallization and preliminary X-ray analysis of the Rhodobacter capsulatus magnesium chelatase BchI subunit

Abstract

Subject classification (UKÄ)

Free keywords

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