TY - JOUR
T1 - Crystallization and preliminary X-ray analysis of tubulin-folding cofactor A from Arabidopsis thaliana
AU - Lu, Lu
AU - Nan, Jie
AU - Mi, Wei
AU - Wei, Chun-Hong
AU - Li, Lan-Fen
AU - Li, Yi
PY - 2010/8/1
Y1 - 2010/8/1
N2 - Tubulin-folding cofactor A (TFC A) is a molecular post-chaperonin that is involved in the beta-tubulin-folding pathway. It has been identified in many organisms including yeasts, humans and plants. In this work, Arabidopsis thaliana TFC A was expressed in Escherichia coli and purified to homogeneity. After thrombin cleavage, a well diffracting crystal was obtained by the sitting-drop vapour-diffusion method at 289 K. The crystal diffracted to 1.6 A resolution using synchrotron radiation and belonged to space group I4(1), with unit-cell parameters a=55.0, b=55.0, c=67.4 A.
AB - Tubulin-folding cofactor A (TFC A) is a molecular post-chaperonin that is involved in the beta-tubulin-folding pathway. It has been identified in many organisms including yeasts, humans and plants. In this work, Arabidopsis thaliana TFC A was expressed in Escherichia coli and purified to homogeneity. After thrombin cleavage, a well diffracting crystal was obtained by the sitting-drop vapour-diffusion method at 289 K. The crystal diffracted to 1.6 A resolution using synchrotron radiation and belonged to space group I4(1), with unit-cell parameters a=55.0, b=55.0, c=67.4 A.
KW - Arabidopsis
KW - Arabidopsis Proteins
KW - Crystallization
KW - Crystallography, X-Ray
KW - Molecular Chaperones
UR - https://www.scopus.com/pages/publications/77955447166
U2 - 10.1107/S1744309110023900
DO - 10.1107/S1744309110023900
M3 - Article
C2 - 20693679
SN - 2053-230X
VL - 66
SP - 954
EP - 956
JO - Acta Crystallographica. Section F: Structural Biology and Crystallization Communications
JF - Acta Crystallographica. Section F: Structural Biology and Crystallization Communications
IS - Pt 8
ER -