TY - JOUR
T1 - Cytochalasin B-induced membrane vesicles convey angiogenic activity of parental cells
AU - Gomzikova, Marina O.
AU - Zhuravleva, Margarita N.
AU - Miftakhova, Regina R.
AU - Arkhipova, Svetlana S.
AU - Evtugin, Vladimir G.
AU - Khaiboullina, Svetlana F.
AU - Kiyasov, Andrey P.
AU - Persson, Jenny L.
AU - Mongan, Nigel P.
AU - Pestell, Richard G.
AU - Rizvanov, Albert
PY - 2017
Y1 - 2017
N2 - Naturally occurring extracellular vesicles (EVs) play essential roles in intracellular communication and delivery of bioactive molecules. Therefore it has been suggested that EVs could be used for delivery of therapeutics. However, to date the therapeutic application of EVs has been limited by number of factors, including limited yield and full understanding of their biological activities. To address these issues, we analyzed the morphology, molecular composition, fusion capacity and biological activity of Cytochalasin B-induced membrane vesicles (CIMVs). The size of these vesicles was comparable to that of naturally occurring EVs. In addition, we have shown that CIMVs from human SH-SY5Y cells contain elevated levels of VEGF as compared to the parental cells, and stimulate angiogenesis in vitro and in vivo.
AB - Naturally occurring extracellular vesicles (EVs) play essential roles in intracellular communication and delivery of bioactive molecules. Therefore it has been suggested that EVs could be used for delivery of therapeutics. However, to date the therapeutic application of EVs has been limited by number of factors, including limited yield and full understanding of their biological activities. To address these issues, we analyzed the morphology, molecular composition, fusion capacity and biological activity of Cytochalasin B-induced membrane vesicles (CIMVs). The size of these vesicles was comparable to that of naturally occurring EVs. In addition, we have shown that CIMVs from human SH-SY5Y cells contain elevated levels of VEGF as compared to the parental cells, and stimulate angiogenesis in vitro and in vivo.
KW - Angiogenesis
KW - Cell-free therapy
KW - Cytochalasin B-induced membrane vesicles
KW - Extracellular vesicles
KW - Membrane vesicles
UR - http://www.scopus.com/inward/record.url?scp=85030257003&partnerID=8YFLogxK
U2 - 10.18632/oncotarget.19723
DO - 10.18632/oncotarget.19723
M3 - Article
C2 - 29050297
AN - SCOPUS:85030257003
SN - 1949-2553
VL - 8
SP - 70496
EP - 70507
JO - Oncotarget
JF - Oncotarget
IS - 41
ER -