Cytoskeletal Regulation During Embryonic Development in Drosophila melanogaster

Mojgan Padash

Research output: ThesisDoctoral Thesis (compilation)


The development of multicellular organisms is associated with extensive rearrangements of cells and tissues. The driving force for these rearrangements is generated by the cell's actin cytoskeleton. During many morphogenetic processes dynamic rearrangement of the actin cytoskeleton is regulated by small GTPases of the Rho-family. These GTPases are highly conserved throughout the animal kingdom and act as bipolar molecular switches that are activated by guanine nucleotide exchange factors (GEFs). The broad tissue distribution of Rho-family GTPases and the presence of a large number of RhoGEFs in the genome have led to the hypothesis that specific aspects of Rho-function might be regulated by specific RhoGEFs in a tissue-specific manner. DRhoGEF2, which is the Drosophila ortholog of the human RGS-domain-containing RhoGEFs, PDZ-RhoGEF, Leukemia associated RhoGEF (LARG) and P115 RhoGEF, has previously been shown to regulate the coordinated cell shape changes that drive the invagination of mesodermal and endodermal germlayers during gastrulation. The tissue distribution of DRhoGEF2 protein suggested a requirement of DRhoGEF2 in a broad range of tissues during embryonic development. This thesis describes the role of DRhoGEF2 during early embryonic development in processes such as metaphase furrow formation, pole cell formation and blastoderm cellularization as well as its later function in the formation of segmental grooves. All processes requiring DRhoGEF2 function involve the contraction of Actin-Myosin fibers and together with previous studies the presented results suggest that DRhoGEF2 regulates the activity of the small GTPase Rho1 during morphogenetic movements that require the contractile activity of actomyosin networks.
Original languageEnglish
Awarding Institution
  • Department of Experimental Medical Science
  • Häcker, Udo, Supervisor
Award date2006 Oct 12
Print ISBNs91-85559-33-4
Publication statusPublished - 2006

Bibliographical note

Defence details

Date: 2006-10-12
Time: 13:00
Place: Segerfalksalen, Wallenberg Neurocentrum, Sölvegatan 17, Lund

External reviewer(s)

Name: Knust, Elisabeth
Title: Professor
Affiliation: Institut für Genetik, Heinrich-Heine Universität Düsseldorf. Germany


<div class="article_info">U Häcker, S Nystedt, M Padash Barmchi, C Horn and E A Wimmer. <span class="article_issue_date">2003</span>. <span class="article_title">piggyBac-based insertional mutagenesis in the presence of stably integrated P elements in Drosophila.</span> <span class="journal_series_title">Proc Natl Acad Sci USA</span>, <span class="journal_volume">vol 100</span> <span class="journal_pages">pp 7720-7725</span>.</div>
<div class="article_info">M Padash Barmchi, S Rogers and U Häcker. <span class="article_issue_date">2005</span>. <span class="article_title">DRhoGEF2 regulates Actin organization and contractility in the Drosophila blastoderm embryo.</span> <span class="journal_series_title">The Journal of Cell Biology</span>, <span class="journal_volume">vol 168</span> <span class="journal_pages">pp 575-585</span>.</div>
<div class="article_info">M Padash Barmchi, S Mulinari and U Häcker. <span class="article_issue_date"></span>. <span class="article_title">DRhoGEF2 is required for segmental groove formation in the Drosophila embryo.</span> (manuscript)</div>

Subject classification (UKÄ)

  • Basic Medicine


  • Naturvetenskap
  • Natural science
  • DRhoGEF2
  • Actin cytoskeleton
  • Rho GTPase


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