D-serine influences synaptogenesis in a P19 cell model

Sabine A. Fuchs, Martin W. Roeleveld, Leo W.J. Klomp, Ruud Berger, Tom J. de Koning

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review

Abstract

Recently, d-serine has been identified as an important NMDA-receptor co-agonist, which might play a role in central nervous system development. We investigated this by studying rat P19 cells, an established model for neuronal and glial differentiation. Our results show that (1) the d-serine synthesizing enzyme serine racemase was expressed upon differentiation, (2) extracellular d-serine concentrations increased upon differentiation, which was inhibited by serine racemase antagonism, and (3) inhibition of d-serine synthesis or prevention of d-serine binding to the NMDA-receptor increased synaptophysin expression and intercellular connections, supporting a role for NMDA-receptor activation by d-serine, synthesized by serine racemase, in shaping synaptogenesis and neuronal circuitry during central nervous system development. In conjunction with recent evidence from literature, we therefore suggest that d-serine deficiency might be responsible for the severe neurological phenotype seen in patients with serine deficiency disorders. In addition, this may provide a pathophysiological mechanism for a role of d-serine deficiency in psychiatric disorders.

Original languageEnglish
Title of host publicationJIMD Reports
PublisherSpringer Gabler
Pages47-53
Number of pages7
DOIs
Publication statusPublished - 2012 Jan 1
Externally publishedYes

Publication series

NameJIMD Reports
Volume6
ISSN (Print)2192-8304
ISSN (Electronic)2192-8312

Subject classification (UKÄ)

  • Neurosciences

Free keywords

  • Central nervous system development
  • Glial fibrillary acidic protein
  • Neuronal circuitry
  • NMDAr activation
  • Serine racemase

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