Decorin deficiency leads to impaired angiogenesis in injured mouse cornea

E Schonherr, C Sunderkotter, L Schaefer, S Thanos, S Grassel, Åke Oldberg, RV Iozzo, MF Young, H Kresse

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Small leucine-rich proteoglycans play important roles in the organization of the extracellular matrix as well as for the regulation of cell behavior; two biological processes that are essential for angiogenesis. We investigated consequences of the targeted ablation of decorin (DCN), biglycan (BGN) and fibromodulin (FMOD) genes on inflammation-induced angiogenesis in the cornea. In wildtype mice, DCN was localized exclusively to the corneal stroma, while FMOD and BGN were more prominently expressed in epithelial cells. Endothelial cells from limbus blood vessels expressed BGN and FMOD, but no DCN. However, after induction of angiogenesis by chemical cauterization, DCN was expressed in the newly formed capillaries, together with BGN and FMOD. Notably, in DCN-deficient mice, the growth of vessels was significantly diminished, whereas it did not significantly change in FMOD- or BGN-deficient animals. Moreover, blood vessels of DCN-deficient mice exhibited a similar expression level of BGN as control mice, while FMOD was increased on day 3 after injury. These results indicate that DCN, in addition to its effects on fibrillogenesis, plays a regulatory role in angiogenesis and that FMOD in endothelial cells may be able to partially substitute for DCN.
    Original languageEnglish
    Pages (from-to)499-508
    JournalJournal of Vascular Research
    Volume41
    Issue number6
    DOIs
    Publication statusPublished - 2004

    Subject classification (UKÄ)

    • Cardiac and Cardiovascular Systems

    Free keywords

    • vascular endothelium
    • extracellular matrix
    • inflammation
    • collagen
    • corneal epithelium

    Fingerprint

    Dive into the research topics of 'Decorin deficiency leads to impaired angiogenesis in injured mouse cornea'. Together they form a unique fingerprint.

    Cite this