Deep learning-enhanced light-field imaging with continuous validation

Nils Wagner, Fynn Beuttenmueller, Nils Norlin, Jakob Gierten, Joachim Wittbrodt, Martin Weigert, Lars Hufnagel, Robert Prevedel, Anna Kreshuk

Research output: Other contributionMiscellaneousResearch

Abstract

Light field microscopy (LFM) has emerged as a powerful tool for fast volumetric image acquisition in biology, but its effective throughput and widespread use has been hampered by a computationally demanding and artefact-prone image reconstruction process. Here, we present a novel framework consisting of a hybrid light-field light-sheet microscope and deep learning-based volume reconstruction, where single light-sheet acquisitions continuously serve as training data and validation for the convolutional neural network reconstructing the LFM volume. Our network delivers high-quality reconstructions at video-rate throughput and we demonstrate the capabilities of our approach by imaging medaka heart dynamics and zebrafish neural activity.
Original languageEnglish
Number of pages24
DOIs
Publication statusSubmitted - 2020 Jul 31

Publication series

NamebioRxiv
PublisherCold Spring Harbor Laboratory Press (CSHL)

Subject classification (UKÄ)

  • Cell and Molecular Biology

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