Degradation of human secretory immunoglobulin A by Blastocystis

Manoj K Puthia; Aparna Vaithilingam; Jia Lu; Kevin S W Tan

doi:10.1007/s00436-005-1461-0

Degradation of human secretory immunoglobulin A by Blastocystis

Manoj K Puthia, Aparna Vaithilingam, Jia Lu, Kevin S W Tan

Research output: Contribution to journal › Article › peer-review

Abstract

Microbial immunoglobulin A (IgA) proteases cleave human secretory IgA, promoting the mucosal adhesion of pathogens. To investigate if the enteric protozoan Blastocystis degrades human secretory IgA, cell lysate and conditioned medium from two species were exposed to immunoglobulin A. Secretory IgA was cleaved by both cell lysate and conditioned medium with mainly cysteine proteinase activity in B. hominis B isolate and aspartic proteinase activity in B. ratii WR1 isolate. These findings suggest that Blastocystis proteases may play a role in parasite survival in vivo.

Original language	English
Pages (from-to)	386-9
Number of pages	4
Journal	Parasitology Research
Volume	97
Issue number	5
DOIs	https://doi.org/10.1007/s00436-005-1461-0
Publication status	Published - 2005 Nov
Externally published	Yes

Subject classification (UKÄ)

Microbiology in the medical area
Immunology in the medical area

Keywords

Animals
Aspartic Acid Endopeptidases/metabolism
Blastocystis/enzymology
Blastocystis hominis/enzymology
Culture Media, Conditioned/metabolism
Cysteine Endopeptidases/metabolism
Humans
Immunoglobulin A, Secretory/metabolism
Rats

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10.1007/s00436-005-1461-0

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title = "Degradation of human secretory immunoglobulin A by Blastocystis",

abstract = "Microbial immunoglobulin A (IgA) proteases cleave human secretory IgA, promoting the mucosal adhesion of pathogens. To investigate if the enteric protozoan Blastocystis degrades human secretory IgA, cell lysate and conditioned medium from two species were exposed to immunoglobulin A. Secretory IgA was cleaved by both cell lysate and conditioned medium with mainly cysteine proteinase activity in B. hominis B isolate and aspartic proteinase activity in B. ratii WR1 isolate. These findings suggest that Blastocystis proteases may play a role in parasite survival in vivo.",

keywords = "Animals, Aspartic Acid Endopeptidases/metabolism, Blastocystis/enzymology, Blastocystis hominis/enzymology, Culture Media, Conditioned/metabolism, Cysteine Endopeptidases/metabolism, Humans, Immunoglobulin A, Secretory/metabolism, Rats",

author = "Puthia, {Manoj K} and Aparna Vaithilingam and Jia Lu and Tan, {Kevin S W}",

year = "2005",

month = nov,

doi = "10.1007/s00436-005-1461-0",

language = "English",

volume = "97",

pages = "386--9",

journal = "Parasitology Research",

issn = "1432-1955",

publisher = "Springer",

number = "5",

}

TY - JOUR

T1 - Degradation of human secretory immunoglobulin A by Blastocystis

AU - Puthia, Manoj K

AU - Vaithilingam, Aparna

AU - Lu, Jia

AU - Tan, Kevin S W

PY - 2005/11

Y1 - 2005/11

N2 - Microbial immunoglobulin A (IgA) proteases cleave human secretory IgA, promoting the mucosal adhesion of pathogens. To investigate if the enteric protozoan Blastocystis degrades human secretory IgA, cell lysate and conditioned medium from two species were exposed to immunoglobulin A. Secretory IgA was cleaved by both cell lysate and conditioned medium with mainly cysteine proteinase activity in B. hominis B isolate and aspartic proteinase activity in B. ratii WR1 isolate. These findings suggest that Blastocystis proteases may play a role in parasite survival in vivo.

AB - Microbial immunoglobulin A (IgA) proteases cleave human secretory IgA, promoting the mucosal adhesion of pathogens. To investigate if the enteric protozoan Blastocystis degrades human secretory IgA, cell lysate and conditioned medium from two species were exposed to immunoglobulin A. Secretory IgA was cleaved by both cell lysate and conditioned medium with mainly cysteine proteinase activity in B. hominis B isolate and aspartic proteinase activity in B. ratii WR1 isolate. These findings suggest that Blastocystis proteases may play a role in parasite survival in vivo.

KW - Animals

KW - Aspartic Acid Endopeptidases/metabolism

KW - Blastocystis/enzymology

KW - Blastocystis hominis/enzymology

KW - Culture Media, Conditioned/metabolism

KW - Cysteine Endopeptidases/metabolism

KW - Humans

KW - Immunoglobulin A, Secretory/metabolism

KW - Rats

U2 - 10.1007/s00436-005-1461-0

DO - 10.1007/s00436-005-1461-0

M3 - Article

C2 - 16151742

VL - 97

SP - 386

EP - 389

JO - Parasitology Research

JF - Parasitology Research

SN - 1432-1955

IS - 5

ER -

Degradation of human secretory immunoglobulin A by Blastocystis

Abstract

Subject classification (UKÄ)

Keywords

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