Abstract
Molecular motors of the cell are protein-based, nanoscale machines, which use a variety of strategies to transduce chemical energy into mechanical work in the presence of a large thermal background. The design and construction of artificial molecular motors is one approach to better understand their basic physical principles. Here, we propose the concept of a protein-based, burnt-bridges ratchet, inspired by biological examples. Our concept, the lawnmower, utilizes protease blades to cleave peptide substrates, and uses the asymmetric substrate-product interface arising from productive cleavage to bias subsequent diffusion on the track (lawn). Following experimental screening to select a protease to act as the motor's blades, we chemically couple trypsin to quantum dots and demonstrate activity of the resulting lawnmower construct in solution. Accompanying Brownian dynamics simulations illustrate the importance for processivity of correct protease density on the quantum dot and spacing of substrates on the track. These results lay the groundwork for future tests of the protein-based lawnmower's motor performance characteristics.
Original language | English |
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Pages (from-to) | 305-312 |
Journal | IEEE Transactions on Nanobioscience |
Volume | 14 |
Issue number | 3 |
DOIs | |
Publication status | Published - 2015 |
Subject classification (UKÄ)
- Nano Technology
Free keywords
- Burnt-bridges
- molecular motor
- protein
- quantum dot
- trypsin