Design of recombinant antibody microarrays for membrane protein profiling of cell lysates and tissue extracts.

Linda Dexlin Mellby, Anna Sandström Gerdtsson, Linn Antberg, Jenny Gunnarsson, Stefan Hansson, Carl Borrebaeck, Christer Wingren

Research output: Contribution to journalArticlepeer-review

Abstract

Generating global protein expression profiles, including also membrane proteins, will be crucial for our understanding of biological processes in health and disease. In this study, we have expanded our antibody microarray technology platform and designed the first human recombinant antibody microarray for membrane proteins targeting crude cell lysates and tissue extracts. We have optimized all key technological parameters and successfully developed a setup for extracting, labeling and analyzing non-fractionated membrane proteomes under non-denaturing conditions. Finally, the platform was also extended and shown to be compatible with simultaneous profiling of both membrane proteins and water-soluble proteins.
Original languageEnglish
Pages (from-to)1550-1554
JournalProteomics
Volume11
DOIs
Publication statusPublished - 2011

Subject classification (UKÄ)

  • Obstetrics, Gynecology and Reproductive Medicine

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