Detailed characterization of plasmids carrying resistance genes using optical DNA mapping

Vilhelm Müller; Lena K. Nyberg; Saair Quaderi; Erik Kristiansson; Tobias Ambjörnsson; Linus Sandegren; Fredrik Westerlund

Detailed characterization of plasmids carrying resistance genes using optical DNA mapping

Vilhelm Müller, Lena K. Nyberg, Saair Quaderi, Erik Kristiansson, Tobias Ambjörnsson, Linus Sandegren, Fredrik Westerlund

Research output: Chapter in Book/Report/Conference proceeding › Paper in conference proceeding › peer-review

Abstract

We present an assay, based on optical DNA mapping in nanochannels that is capable of characterizing the plasmid content of bacterial isolates resistant to antibiotics in a fast an detailed way. In a single experiment we determine the number of different plasmids in each sample, their size, as well as a barcode that can be used for plasmid identification and tracing. In addition we demonstrate that we can identify resistance genes on individual plasmids using CRISPR/Cas9. We foresee that the assay can be a useful tool all the way from fundamental plasmid biology to diagnostics and surveillance of resistant infections.

Original language	English
Title of host publication	20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
Publisher	Chemical and Biological Microsystems Society
Pages	1561-1562
Number of pages	2
ISBN (Electronic)	9780979806490
Publication status	Published - 2016
Event	20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 - Dublin, Ireland Duration: 2016 Oct 9 → 2016 Oct 13

Conference

Conference	20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
Country/Territory	Ireland
City	Dublin
Period	2016/10/09 → 2016/10/13

Subject classification (UKÄ)

Medical Laboratory Technologies
Biophysics
Other Physics Topics

Free keywords

Antibiotic resistance
CRISPR/Cas9
Nanofluidics
Optical DNA mapping
Plasmids

Cite this

Müller, V., Nyberg, L. K., Quaderi, S., Kristiansson, E., Ambjörnsson, T., Sandegren, L., & Westerlund, F. (2016). Detailed characterization of plasmids carrying resistance genes using optical DNA mapping. In 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 (pp. 1561-1562). Chemical and Biological Microsystems Society.

@inproceedings{d446b5e366ab4c1286b9e88fd363ba7b,

title = "Detailed characterization of plasmids carrying resistance genes using optical DNA mapping",

abstract = "We present an assay, based on optical DNA mapping in nanochannels that is capable of characterizing the plasmid content of bacterial isolates resistant to antibiotics in a fast an detailed way. In a single experiment we determine the number of different plasmids in each sample, their size, as well as a barcode that can be used for plasmid identification and tracing. In addition we demonstrate that we can identify resistance genes on individual plasmids using CRISPR/Cas9. We foresee that the assay can be a useful tool all the way from fundamental plasmid biology to diagnostics and surveillance of resistant infections.",

keywords = "Antibiotic resistance, CRISPR/Cas9, Nanofluidics, Optical DNA mapping, Plasmids",

author = "Vilhelm M{\"u}ller and Nyberg, {Lena K.} and Saair Quaderi and Erik Kristiansson and Tobias Ambj{\"o}rnsson and Linus Sandegren and Fredrik Westerlund",

year = "2016",

language = "English",

pages = "1561--1562",

booktitle = "20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016",

publisher = "Chemical and Biological Microsystems Society",

note = "20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 ; Conference date: 09-10-2016 Through 13-10-2016",

}

Müller, V, Nyberg, LK, Quaderi, S, Kristiansson, E, Ambjörnsson, T, Sandegren, L & Westerlund, F 2016, Detailed characterization of plasmids carrying resistance genes using optical DNA mapping. in 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society, pp. 1561-1562, 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016, Dublin, Ireland, 2016/10/09.

Detailed characterization of plasmids carrying resistance genes using optical DNA mapping. / Müller, Vilhelm; Nyberg, Lena K.; Quaderi, Saair et al.
20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society, 2016. p. 1561-1562.

Research output: Chapter in Book/Report/Conference proceeding › Paper in conference proceeding › peer-review

TY - GEN

T1 - Detailed characterization of plasmids carrying resistance genes using optical DNA mapping

AU - Müller, Vilhelm

AU - Nyberg, Lena K.

AU - Quaderi, Saair

AU - Kristiansson, Erik

AU - Ambjörnsson, Tobias

AU - Sandegren, Linus

AU - Westerlund, Fredrik

PY - 2016

Y1 - 2016

N2 - We present an assay, based on optical DNA mapping in nanochannels that is capable of characterizing the plasmid content of bacterial isolates resistant to antibiotics in a fast an detailed way. In a single experiment we determine the number of different plasmids in each sample, their size, as well as a barcode that can be used for plasmid identification and tracing. In addition we demonstrate that we can identify resistance genes on individual plasmids using CRISPR/Cas9. We foresee that the assay can be a useful tool all the way from fundamental plasmid biology to diagnostics and surveillance of resistant infections.

AB - We present an assay, based on optical DNA mapping in nanochannels that is capable of characterizing the plasmid content of bacterial isolates resistant to antibiotics in a fast an detailed way. In a single experiment we determine the number of different plasmids in each sample, their size, as well as a barcode that can be used for plasmid identification and tracing. In addition we demonstrate that we can identify resistance genes on individual plasmids using CRISPR/Cas9. We foresee that the assay can be a useful tool all the way from fundamental plasmid biology to diagnostics and surveillance of resistant infections.

KW - Antibiotic resistance

KW - CRISPR/Cas9

KW - Nanofluidics

KW - Optical DNA mapping

KW - Plasmids

UR - http://www.scopus.com/inward/record.url?scp=85014171223&partnerID=8YFLogxK

M3 - Paper in conference proceeding

AN - SCOPUS:85014171223

SP - 1561

EP - 1562

BT - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016

PB - Chemical and Biological Microsystems Society

T2 - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016

Y2 - 9 October 2016 through 13 October 2016

ER -

Detailed characterization of plasmids carrying resistance genes using optical DNA mapping

Abstract

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Subject classification (UKÄ)

Free keywords

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