Development and Application of Enzyme- and Antibody-based Assays with Emphasis on Selectivity, Sensitivity and Stability

Catalin Nistor

Development and Application of Enzyme- and Antibody-based Assays with Emphasis on Selectivity, Sensitivity and Stability

Catalin Nistor

Centre for Analysis and Synthesis

Research output: Thesis › Doctoral Thesis (compilation)

Abstract

This thesis presents the development, optimisation, and various applications of enzyme-based biosensors and immunoassays, mainly with relevance to environmental analysis. the thesis discusses problems and possible solutions associated with selectivity, sensitivity and robustness of such bioassays with emphasis on various immunoasssay configurations for determination of surfactants, phenols, or drugs, and amperometric biosensors based on enzymes (e.g., tyrosinase, cellobiose dehydrogenase, and glucose dehydrogenase), applied for the determination of phenols from environmental samples, or as selective detectors in flow immunoassay.

Original language	English
Qualification	Doctor
Awarding Institution	Centre for Analysis and Synthesis
Supervisors/Advisors	[unknown], [unknown], Supervisor, External person
Award date	2002 Sept 5
Publisher	Catalin Nistor, Department of Analytical Chemistry, Lund University
ISBN (Print)	91-7422-006-3
Publication status	Published - 2002

Bibliographical note

Defence details

Date: 2002-09-05
Time: 10:15
Place: Center of Chemistry and Chemical Engineering, Lecture Hall B

External reviewer(s)

Name: Irth, Hubertus
Title: Professor
Affiliation: Division of Analytical Chemistry, Vrije University, amsterdam, The Netherlands

---

Article: I. C. Nistor, J. Emnéus, L. Gorton, and A. Ciucu, "Improved stability and altered selectivity of tyrosinase-based carbon electrodes for detection of phenolic compounds", Anal. Chim. Acta, 1999, 387, 309-326.

Article: II. C. Nistor, A. Oubiña, D. Barceló, M.-P. Marco, and J. Emnéus, "Competitive flow immunoassay with fluorescence detection for determination of 4-nitrophenol", Anal. Chim. Acta, 2001, 426, 185-195.

Article: III. E. Burestedt, C. Nistor, U. Schagerlöf, and J. Emnéus, "An enzyme-flow immunoassay that uses b-galactosidase as the label and a cellobiose dehydrogenase biosensor as the label detector", Anal. Chem., 2000, 72, 4171-4177.

Article: IV. M. Badea, C. Nistor, Y. Goda, S. Fujimoto, S. Dosho, A. Danet, D. Barceló, F. Ventura, and J. Emnéus, "A flow immunoassay for alkylphenol ethoxylate surfactants?highlighting questions associated with cross-reactivity, matrix effects, and validation by chromatographic techniques", 2002, submitted to Anal. Chem.

Article: V. C. Nistor and J. Emnéus, "A capillary-based amperometric flow-immunoassay for 2,4,6-trichlorophenol", 2002, submitted to Anal. Bioanal. Chem.

Article: VI. M. Fránek, J. Zeravík, S. Eremin, J. Yakovleva, M. Badea, A. Danet, N. Ocio, C. Nistor, and J. Emnéus, "Antibody-based methods for surfactant screening", Fresenius? J. Anal. Chem., 2001, 371, 456-466.

Article: VII. C. Nistor, M.-P. Marco, M. Tudorache, and J. Emnéus, "Practical ways to modulate the sensitivity of competitive immunoassays", 2002, submitted to Anal. Biochem.

Article: VIII. C. Nistor and J. Emnéus, "An enzyme flow immunoassay using alkaline phosphatase as the label and a tyrosinase biosensor as the label detector", Anal. Commun., 1998, 35, 417-419.

Article: IX. C. Nistor, A. Rose, M. Farré, L. Stoica, U. Wollenberger, T. Ruzgas, D. Pfeiffer, D. Barceló, L. Gorton, and J. Emnéus, "In-field monitoring of cleaning efficiency in wastewater treatment plants using two phenol-sensitive biosensors", Anal. Chim. Acta, 2002, 456, 3-17.

Article: X. C. Nistor, J. Christensen, N. Ocio, L. Nørgaard, and J. Emnéus, "Multivariate analysis to resolve the signal given by cross-reactants in immunoassay with sample matrix dilution", 2002, submitted to Anal. Chem.

Article: XI. C. Nistor, A. Osvik, R. Davidsson, A. Rose, U. Wollenberger, D. Pfeiffer, J. Emnéus and L. Fiksdal, "Detection of Escherichia coli in water by culture-based amperometric and luminometric methods", Water Sci. Technol., 2002, 45, 191-199.

Article: XII. M. Castillo, J. Riu, F. Ventura, R. Boleda, R. Scheding, H. Fr. Schröder, C. Nistor, J. Emnéus, P. Eichhorn, Th. P. Knepper, C.C.A. Jonkers, P. de Voogt, E. González-Mazo, V.M. Léon, and D. Barceló, "Inter-laboratory comparison of liquid chromatographic techniques and enzyme-linked immunosorbent assay for the determination of surfactants in wastewaters", J. Chromatogr. A, 2000, 889, 195-209.

The information about affiliations in this record was updated in December 2015.
The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)

Subject classification (UKÄ)

Analytical Chemistry

Free keywords

stability
sensitivity
selectivity
cross-reactivity
sample matrix effects
environmental pollutants
enzyme biosensor
immunoassay
Analytical chemistry
Analytisk kemi

Cite this

@phdthesis{5db78894fea6400fbc51fbea2051b01a,

title = "Development and Application of Enzyme- and Antibody-based Assays with Emphasis on Selectivity, Sensitivity and Stability",

abstract = "This thesis presents the development, optimisation, and various applications of enzyme-based biosensors and immunoassays, mainly with relevance to environmental analysis. the thesis discusses problems and possible solutions associated with selectivity, sensitivity and robustness of such bioassays with emphasis on various immunoasssay configurations for determination of surfactants, phenols, or drugs, and amperometric biosensors based on enzymes (e.g., tyrosinase, cellobiose dehydrogenase, and glucose dehydrogenase), applied for the determination of phenols from environmental samples, or as selective detectors in flow immunoassay.",

keywords = "stability, sensitivity, selectivity, cross-reactivity, sample matrix effects, environmental pollutants, enzyme biosensor, immunoassay, Analytical chemistry, Analytisk kemi",

author = "Catalin Nistor",

note = "Defence details Date: 2002-09-05 Time: 10:15 Place: Center of Chemistry and Chemical Engineering, Lecture Hall B External reviewer(s) Name: Irth, Hubertus Title: Professor Affiliation: Division of Analytical Chemistry, Vrije University, amsterdam, The Netherlands --- Article: I. C. Nistor, J. Emn{\'e}us, L. Gorton, and A. Ciucu, {"}Improved stability and altered selectivity of tyrosinase-based carbon electrodes for detection of phenolic compounds{"}, Anal. Chim. Acta, 1999, 387, 309-326. Article: II. C. Nistor, A. Oubi{\~n}a, D. Barcel{\'o}, M.-P. Marco, and J. Emn{\'e}us, {"}Competitive flow immunoassay with fluorescence detection for determination of 4-nitrophenol{"}, Anal. Chim. Acta, 2001, 426, 185-195. Article: III. E. Burestedt, C. Nistor, U. Schagerl{\"o}f, and J. Emn{\'e}us, {"}An enzyme-flow immunoassay that uses b-galactosidase as the label and a cellobiose dehydrogenase biosensor as the label detector{"}, Anal. Chem., 2000, 72, 4171-4177. Article: IV. M. Badea, C. Nistor, Y. Goda, S. Fujimoto, S. Dosho, A. Danet, D. Barcel{\'o}, F. Ventura, and J. Emn{\'e}us, {"}A flow immunoassay for alkylphenol ethoxylate surfactants?highlighting questions associated with cross-reactivity, matrix effects, and validation by chromatographic techniques{"}, 2002, submitted to Anal. Chem. Article: V. C. Nistor and J. Emn{\'e}us, {"}A capillary-based amperometric flow-immunoassay for 2,4,6-trichlorophenol{"}, 2002, submitted to Anal. Bioanal. Chem. Article: VI. M. Fr{\'a}nek, J. Zerav{\'i}k, S. Eremin, J. Yakovleva, M. Badea, A. Danet, N. Ocio, C. Nistor, and J. Emn{\'e}us, {"}Antibody-based methods for surfactant screening{"}, Fresenius? J. Anal. Chem., 2001, 371, 456-466. Article: VII. C. Nistor, M.-P. Marco, M. Tudorache, and J. Emn{\'e}us, {"}Practical ways to modulate the sensitivity of competitive immunoassays{"}, 2002, submitted to Anal. Biochem. Article: VIII. C. Nistor and J. Emn{\'e}us, {"}An enzyme flow immunoassay using alkaline phosphatase as the label and a tyrosinase biosensor as the label detector{"}, Anal. Commun., 1998, 35, 417-419. Article: IX. C. Nistor, A. Rose, M. Farr{\'e}, L. Stoica, U. Wollenberger, T. Ruzgas, D. Pfeiffer, D. Barcel{\'o}, L. Gorton, and J. Emn{\'e}us, {"}In-field monitoring of cleaning efficiency in wastewater treatment plants using two phenol-sensitive biosensors{"}, Anal. Chim. Acta, 2002, 456, 3-17. Article: X. C. Nistor, J. Christensen, N. Ocio, L. N{\o}rgaard, and J. Emn{\'e}us, {"}Multivariate analysis to resolve the signal given by cross-reactants in immunoassay with sample matrix dilution{"}, 2002, submitted to Anal. Chem. Article: XI. C. Nistor, A. Osvik, R. Davidsson, A. Rose, U. Wollenberger, D. Pfeiffer, J. Emn{\'e}us and L. Fiksdal, {"}Detection of Escherichia coli in water by culture-based amperometric and luminometric methods{"}, Water Sci. Technol., 2002, 45, 191-199. Article: XII. M. Castillo, J. Riu, F. Ventura, R. Boleda, R. Scheding, H. Fr. Schr{\"o}der, C. Nistor, J. Emn{\'e}us, P. Eichhorn, Th. P. Knepper, C.C.A. Jonkers, P. de Voogt, E. Gonz{\'a}lez-Mazo, V.M. L{\'e}on, and D. Barcel{\'o}, {"}Inter-laboratory comparison of liquid chromatographic techniques and enzyme-linked immunosorbent assay for the determination of surfactants in wastewaters{"}, J. Chromatogr. A, 2000, 889, 195-209. The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)",

year = "2002",

language = "English",

isbn = "91-7422-006-3",

publisher = "Catalin Nistor, Department of Analytical Chemistry, Lund University",

type = "Doctoral Thesis (compilation)",

school = "Centre for Analysis and Synthesis",

}

TY - THES

T1 - Development and Application of Enzyme- and Antibody-based Assays with Emphasis on Selectivity, Sensitivity and Stability

AU - Nistor, Catalin

N1 - Defence details Date: 2002-09-05 Time: 10:15 Place: Center of Chemistry and Chemical Engineering, Lecture Hall B External reviewer(s) Name: Irth, Hubertus Title: Professor Affiliation: Division of Analytical Chemistry, Vrije University, amsterdam, The Netherlands --- Article: I. C. Nistor, J. Emnéus, L. Gorton, and A. Ciucu, "Improved stability and altered selectivity of tyrosinase-based carbon electrodes for detection of phenolic compounds", Anal. Chim. Acta, 1999, 387, 309-326. Article: II. C. Nistor, A. Oubiña, D. Barceló, M.-P. Marco, and J. Emnéus, "Competitive flow immunoassay with fluorescence detection for determination of 4-nitrophenol", Anal. Chim. Acta, 2001, 426, 185-195. Article: III. E. Burestedt, C. Nistor, U. Schagerlöf, and J. Emnéus, "An enzyme-flow immunoassay that uses b-galactosidase as the label and a cellobiose dehydrogenase biosensor as the label detector", Anal. Chem., 2000, 72, 4171-4177. Article: IV. M. Badea, C. Nistor, Y. Goda, S. Fujimoto, S. Dosho, A. Danet, D. Barceló, F. Ventura, and J. Emnéus, "A flow immunoassay for alkylphenol ethoxylate surfactants?highlighting questions associated with cross-reactivity, matrix effects, and validation by chromatographic techniques", 2002, submitted to Anal. Chem. Article: V. C. Nistor and J. Emnéus, "A capillary-based amperometric flow-immunoassay for 2,4,6-trichlorophenol", 2002, submitted to Anal. Bioanal. Chem. Article: VI. M. Fránek, J. Zeravík, S. Eremin, J. Yakovleva, M. Badea, A. Danet, N. Ocio, C. Nistor, and J. Emnéus, "Antibody-based methods for surfactant screening", Fresenius? J. Anal. Chem., 2001, 371, 456-466. Article: VII. C. Nistor, M.-P. Marco, M. Tudorache, and J. Emnéus, "Practical ways to modulate the sensitivity of competitive immunoassays", 2002, submitted to Anal. Biochem. Article: VIII. C. Nistor and J. Emnéus, "An enzyme flow immunoassay using alkaline phosphatase as the label and a tyrosinase biosensor as the label detector", Anal. Commun., 1998, 35, 417-419. Article: IX. C. Nistor, A. Rose, M. Farré, L. Stoica, U. Wollenberger, T. Ruzgas, D. Pfeiffer, D. Barceló, L. Gorton, and J. Emnéus, "In-field monitoring of cleaning efficiency in wastewater treatment plants using two phenol-sensitive biosensors", Anal. Chim. Acta, 2002, 456, 3-17. Article: X. C. Nistor, J. Christensen, N. Ocio, L. Nørgaard, and J. Emnéus, "Multivariate analysis to resolve the signal given by cross-reactants in immunoassay with sample matrix dilution", 2002, submitted to Anal. Chem. Article: XI. C. Nistor, A. Osvik, R. Davidsson, A. Rose, U. Wollenberger, D. Pfeiffer, J. Emnéus and L. Fiksdal, "Detection of Escherichia coli in water by culture-based amperometric and luminometric methods", Water Sci. Technol., 2002, 45, 191-199. Article: XII. M. Castillo, J. Riu, F. Ventura, R. Boleda, R. Scheding, H. Fr. Schröder, C. Nistor, J. Emnéus, P. Eichhorn, Th. P. Knepper, C.C.A. Jonkers, P. de Voogt, E. González-Mazo, V.M. Léon, and D. Barceló, "Inter-laboratory comparison of liquid chromatographic techniques and enzyme-linked immunosorbent assay for the determination of surfactants in wastewaters", J. Chromatogr. A, 2000, 889, 195-209. The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)

PY - 2002

Y1 - 2002

N2 - This thesis presents the development, optimisation, and various applications of enzyme-based biosensors and immunoassays, mainly with relevance to environmental analysis. the thesis discusses problems and possible solutions associated with selectivity, sensitivity and robustness of such bioassays with emphasis on various immunoasssay configurations for determination of surfactants, phenols, or drugs, and amperometric biosensors based on enzymes (e.g., tyrosinase, cellobiose dehydrogenase, and glucose dehydrogenase), applied for the determination of phenols from environmental samples, or as selective detectors in flow immunoassay.

AB - This thesis presents the development, optimisation, and various applications of enzyme-based biosensors and immunoassays, mainly with relevance to environmental analysis. the thesis discusses problems and possible solutions associated with selectivity, sensitivity and robustness of such bioassays with emphasis on various immunoasssay configurations for determination of surfactants, phenols, or drugs, and amperometric biosensors based on enzymes (e.g., tyrosinase, cellobiose dehydrogenase, and glucose dehydrogenase), applied for the determination of phenols from environmental samples, or as selective detectors in flow immunoassay.

KW - stability

KW - sensitivity

KW - selectivity

KW - cross-reactivity

KW - sample matrix effects

KW - environmental pollutants

KW - enzyme biosensor

KW - immunoassay

KW - Analytical chemistry

KW - Analytisk kemi

M3 - Doctoral Thesis (compilation)

SN - 91-7422-006-3

PB - Catalin Nistor, Department of Analytical Chemistry, Lund University

ER -

Development and Application of Enzyme- and Antibody-based Assays with Emphasis on Selectivity, Sensitivity and Stability

Abstract

Bibliographical note

Subject classification (UKÄ)

Free keywords

Fingerprint

Cite this