Development of a Bioelectronic Tongue -Applications for Wastewater Analysis

Eva Dock

Research output: ThesisDoctoral Thesis (compilation)

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A bioelectronic tongue has been developed for applications in wastewater analysis. The development of a biosensor array with complex signal analysis started from the idea of using group-selective phenol biosensors (tyrosinase and horseradish peroxidase) with chemometric analysis for signal processing. In a first step the ability to simultaneously determine each analyte in synthetic binary phenol mixtures was evaluated using multivariate data analysis on the responses from a single tyrosinase-modified solid graphite electrode. The next step was to construct a suitable device where different biosensors could be used in an array for multi-parameter detection of samples. Screen-printed electrodes of carbon and noble metals were first evaluated with the purpose of identifying electrodes that could be used as a basis for immobilisation of phenol- and pesticide-sensitive enzymes (horseradish peroxidase, soybean peroxidase, cellobiose dehydrogenase, acetylcholinesterase and butyrylcholinesterase). These enzymes were then immobilised on an array of eight radially distributed electrodes. To host the array and to provide equal hydrodynamics at each electrode in the array, a special electrochemical cell was constructed to enable flow-injection and steady-state measurements. Together with multivariate data analysis has this array system been successfully used for qualitative discrimination of wastewater samples as well as for quantitative determination of their toxicity and other pollution parameters such as chemical oxygen demand and biological oxygen demand. Pre-processing of data before multivariate analysis was shown to be necessary for reducing the noise that otherwise can hide the desired structural information. Several strategies to overcome noise problems due to drift in biosensors have been developed.
Original languageEnglish
Awarding Institution
  • Centre for Analysis and Synthesis
  • Ruzgas, Tautgirdas, Supervisor
  • Emnéus, Jenny, Supervisor
Award date2006 Jun 2
ISBN (Print)91-628-6854-3
Publication statusPublished - 2006

Bibliographical note

Defence details

Date: 2006-06-02
Time: 13:15
Place: Lunds universitet, Kemicentrum, Sölvegatan 39, hörsal A

External reviewer(s)

Name: Winquist, Fredrik
Title: Professor
Affiliation: S-SENCE and Laboratory of Applied Physics, Linköpings Universitet, Sverige


<div class="article_info">Eva Dock, Jakob Christensen, Mattias Olsson, Erik Tonning, Tautgirdas Ruzgas and Jenny Emnéus. <span class="article_issue_date">2005</span>. <a href="javascript:downloadFile(546832)" class="article_link">Multivariate Data Analysis of Dynamic Amperometric Biosensor Responses from Binary Analyte Mixtures - Application of Sensitivity Correction Algorithms</a> <span class="journal_series_title">Talanta</span>, <span class="journal_volume">vol 65</span> <span class="journal_pages">pp 298-305</span>. <span class="journal_distributor">Elsevier</span></div>
<div class="article_info">Eva Dock, Andreas Christenson, Svetlana Sapelnikova, Jan Krejci, Jenny Emnéus and Tautgirdas Ruzgas. <span class="article_issue_date">2005</span>. <a href="javascript:downloadFile(546833)" class="article_link">A Steady-State and Flow-Through Cell for Screen-Printed Eight-Electrode Arrays</a> <span class="journal_series_title">Analytica Chimica Acta</span>, <span class="journal_volume">vol 531</span> <span class="journal_pages">pp 165-172</span>. <span class="journal_distributor">Elsevier</span></div>
<div class="article_info">Renata Solná, Eva Dock, Andreas Christenson, Margrethe Winther-Nielsen, Charlotte Carlsson, Jenny Emnéus, Tautgirdas Ruzgas and Petr Skládal. <span class="article_issue_date">2005</span>. <a href="javascript:downloadFile(546834)" class="article_link">Amperometric Screen-Printed Biosensor Arrays with Co-Immobilised Oxidoreductases and Cholinesterases</a> <span class="journal_series_title">Analytica Chimica Acta</span>, <span class="journal_volume">vol 528</span> <span class="journal_pages">pp 9-19</span>. <span class="journal_distributor">Elsevier</span></div>
<div class="article_info">Erik Tonning, Svetlana Sapelnikova, Jakob Christensen, Charlotte Carlsson, Margrethe Winther-Nielsen, Eva Dock, Renata Solná, Petr Skládal, Lars Norgaard, Tautgirdas Ruzgas and Jenny Emnéus. <span class="article_issue_date">2005</span>. <a href="javascript:downloadFile(546835)" class="article_link">Chemometric Exploration of an Amperometric Biosensor Array for Fast Determination of Wastewater Quality</a> <span class="journal_series_title">Biosensors and Bioelectronics</span>, <span class="journal_volume">vol 21</span> <span class="journal_pages">pp 608-617</span>. <span class="journal_distributor">Elsevier</span></div>
<div class="article_info">Eva Dock, Annika Lindgren, Tautgirdas Ruzgas and Lo Gorton. <span class="article_issue_date">2001</span>. <span class="article_title">Effect of Interfering Substances on Current Response of Recombinant Peroxidase and Glucose Oxidase-Recombinant Peroxidase Modified Graphite Electrodes</span> <span class="journal_series_title">Analyst</span>, <span class="journal_volume">vol 126</span> <span class="journal_pages">pp 1929-1935</span>. <span class="journal_distributor">The Royal Society of Chemistry</span></div>
<div class="article_info">Eva Dock and Tautgirdas Ruzgas. <span class="article_issue_date">2003</span>. <span class="article_title">Screen-Printed Carbon Electrodes Modified with Cellobiose Dehydrogenase: Amplification Factor for Catechol vs. Reversibility of Ferricyanide</span> <span class="journal_series_title">Electroanalysis</span>, <span class="journal_volume">vol 15</span> <span class="journal_pages">pp 492-498</span>. <span class="journal_distributor">Wiley</span></div>
<div class="article_info">Eva Dock, Erik Tonning, Jakob Christensen, Margrethe Winther-Nielsen, Charlotte Carlsson, Renata Solná, Petr Skládal, Lars Norgaard, Jenny Emnéus and Tautgirdas Ruzgas. <span class="article_issue_date">2006</span>. <span class="article_title">An amperometric bioelectronic tongue for simultaneous determination of toxicity, COD and BOD in wastewater</span> (manuscript)</div>

The information about affiliations in this record was updated in December 2015.
The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)

Subject classification (UKÄ)

  • Analytical Chemistry


  • Analytisk kemi
  • screen-printed electrode
  • multivariate data analysis
  • wastewater
  • Analytical chemistry
  • electronic tongue
  • biosensor array
  • amperometric


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