Abstract
This paper describes a new amperometric biosensor for glucose monitoring. The biosensor is based on the activity of glucose dehydrogenase (GDH) and diaphorase (DI) co-immobilized with NAD(+) into a carbon nanotube paste (CNTP) electrode modified with an osmium functionalized polymer. This mediator was demonstrated to shuttle the electron transfer between the immobilized diaphorase and the CNTP electrode, thus, showing a good electrocatalytic activity towards NADH oxidation at potentials around +0.2 V versus Ag vertical bar AgCl, where interfering reactions are less prone to occur. The biosensor exhibits a detection limit of 10 mu mol L-1, linearity up to 8 x 10(-4) mol L-1, a sensitivity of 13.4 mu A cm(-2) mmol(-1) L-1, a good reproducibility (R.S.D. 2.1%, n = 6) and a stability of about 1 week when stored dry at 4 degrees C. Finally, the proposed biosensor was applied for the determination of glucose in different samples of sweet wine and validated with a commercial spectrophotometric enzymatic kit. (c) 2006 Elsevier B.V. All rights reserved.
Original language | English |
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Pages (from-to) | 2611-2617 |
Journal | Biosensors & Bioelectronics |
Volume | 22 |
Issue number | 11 |
DOIs | |
Publication status | Published - 2007 |
Bibliographical note
The information about affiliations in this record was updated in December 2015.The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)
Subject classification (UKÄ)
- Analytical Chemistry
Free keywords
- osmium redox
- diaphorase
- glucose dehydrogenase
- biosensor
- glucose
- polymer
- carbon nanotube paste electrode