Development of an amperometric biosensor based on acetylcholine esterase covalently bound to a new support material.

M Khayyami, M T Pérez Pita, N Peña Garcia, Gillis Johansson, Bengt Danielsson, Per-Olof Larsson

Research output: Contribution to journalArticlepeer-review

Abstract

A new type of amperometric biosensor based on immobilised acetylcholine esterase was designed and constructed. The enzyme was immobilised on a flow-through working electrode, which was prepared from reticulated vitreous carbon (RVC) or from a composite material consisting of RVC and superporous agarose. The sensor was operated in FIA mode using acetylthiocholine as a substrate. The sensor responded to inhibitors such as paraoxon-10(-9) mol was detected by the sensor in a non-optimised configuration. The practical lifetime of the sensor was at least 1 month.
Original languageEnglish
Pages (from-to)557-563
JournalTalanta
Volume45
Issue number3
DOIs
Publication statusPublished - 1998

Bibliographical note

The information about affiliations in this record was updated in December 2015.
The record was previously connected to the following departments: Pure and Applied Biochemistry (LTH) (011001005), Analytical Chemistry (S/LTH) (011001004)

Subject classification (UKÄ)

  • Analytical Chemistry
  • Biochemistry and Molecular Biology

Keywords

  • Acetylthiocholine
  • Acetylcholine esterase
  • Biosensor
  • RVC-electrode
  • Amperometric
  • Thiocholine
  • Mediator
  • Superporous agarose gel

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