Development of an amperometric biosensor based on acetylcholine esterase covalently bound to a new support material

M Khayyami; M T Pérez Pita; N Peña Garcia; Gillis Johansson; Bengt Danielsson; Per-Olof Larsson

doi:10.1016/S0039-9140(97)00182-3

Development of an amperometric biosensor based on acetylcholine esterase covalently bound to a new support material

M Khayyami, M T Pérez Pita, N Peña Garcia, Gillis Johansson, Bengt Danielsson, Per-Olof Larsson

Research output: Contribution to journal › Article › peer-review

Abstract

A new type of amperometric biosensor based on immobilised acetylcholine esterase was designed and constructed. The enzyme was immobilised on a flow-through working electrode, which was prepared from reticulated vitreous carbon (RVC) or from a composite material consisting of RVC and superporous agarose. The sensor was operated in FIA mode using acetylthiocholine as a substrate. The sensor responded to inhibitors such as paraoxon—10−9 mol was detected by the sensor in a non-optimised configuration. The practical lifetime of the sensor was at least 1 month.

Original language	English
Pages (from-to)	557-563
Journal	Talanta
Volume	45
Issue number	3
DOIs	https://doi.org/10.1016/S0039-9140(97)00182-3
Publication status	Published - 1998

Subject classification (UKÄ)

Composite Science and Engineering
Analytical Chemistry

Free keywords

Acetylthiocholine
Acetylcholine esterase
Biosensor
RVC-electrode
Amperometric
Thiocholine
Mediator
Superporous agarose gel

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10.1016/S0039-9140(97)00182-3

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title = "Development of an amperometric biosensor based on acetylcholine esterase covalently bound to a new support material",

abstract = "A new type of amperometric biosensor based on immobilised acetylcholine esterase was designed and constructed. The enzyme was immobilised on a flow-through working electrode, which was prepared from reticulated vitreous carbon (RVC) or from a composite material consisting of RVC and superporous agarose. The sensor was operated in FIA mode using acetylthiocholine as a substrate. The sensor responded to inhibitors such as paraoxon—10−9 mol was detected by the sensor in a non-optimised configuration. The practical lifetime of the sensor was at least 1 month.",

keywords = "Acetylthiocholine, Acetylcholine esterase, Biosensor, RVC-electrode, Amperometric, Thiocholine, Mediator, Superporous agarose gel",

author = "M Khayyami and \{P{\'e}rez Pita\}, \{M T\} and \{Pe{\~n}a Garcia\}, N and Gillis Johansson and Bengt Danielsson and Per-Olof Larsson",

year = "1998",

doi = "10.1016/S0039-9140(97)00182-3",

language = "English",

volume = "45",

pages = "557--563",

journal = "Talanta",

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T1 - Development of an amperometric biosensor based on acetylcholine esterase covalently bound to a new support material

AU - Khayyami, M

AU - Pérez Pita, M T

AU - Peña Garcia, N

AU - Johansson, Gillis

AU - Danielsson, Bengt

AU - Larsson, Per-Olof

PY - 1998

Y1 - 1998

N2 - A new type of amperometric biosensor based on immobilised acetylcholine esterase was designed and constructed. The enzyme was immobilised on a flow-through working electrode, which was prepared from reticulated vitreous carbon (RVC) or from a composite material consisting of RVC and superporous agarose. The sensor was operated in FIA mode using acetylthiocholine as a substrate. The sensor responded to inhibitors such as paraoxon—10−9 mol was detected by the sensor in a non-optimised configuration. The practical lifetime of the sensor was at least 1 month.

AB - A new type of amperometric biosensor based on immobilised acetylcholine esterase was designed and constructed. The enzyme was immobilised on a flow-through working electrode, which was prepared from reticulated vitreous carbon (RVC) or from a composite material consisting of RVC and superporous agarose. The sensor was operated in FIA mode using acetylthiocholine as a substrate. The sensor responded to inhibitors such as paraoxon—10−9 mol was detected by the sensor in a non-optimised configuration. The practical lifetime of the sensor was at least 1 month.

KW - Acetylthiocholine

KW - Acetylcholine esterase

KW - Biosensor

KW - RVC-electrode

KW - Amperometric

KW - Thiocholine

KW - Mediator

KW - Superporous agarose gel

UR - https://www.scopus.com/pages/publications/0031941469

U2 - 10.1016/S0039-9140(97)00182-3

DO - 10.1016/S0039-9140(97)00182-3

M3 - Article

SN - 1873-3573

VL - 45

SP - 557

EP - 563

JO - Talanta

JF - Talanta

IS - 3

ER -

Development of an amperometric biosensor based on acetylcholine esterase covalently bound to a new support material

Abstract

Subject classification (UKÄ)

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