Abstract
Considering the widespread contaminations of food products with mycotoxins, it is important to develop, robust, time- and cost-effective detection methods. We developed and optimized an immunoassay using a continuous flow system for the detection of zearalenone (ZEN). The assay was performed in a flow mode using an automated sequential injection system. Time for an assay cycle was 18 Min. Under optimal conditions, the limit for quantification for ZEN was 0.40 µg L−1 with a linear dependency between concentration and signal amplitude between 0.10 and 10 µg L−1. The assay proved to be robust and reliable with 13% relative standard deviation between measurements. By dissociating the antigen–antibody complex using a regeneration solution, we showed 50 times reusability of the immobilized antibodies without affecting the antigen-binding properties.
Original language | English |
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Pages (from-to) | 375-382 |
Number of pages | 8 |
Journal | Biotechnology and Applied Biochemistry |
Volume | 67 |
Issue number | 3 |
DOIs | |
Publication status | Published - 2020 May 1 |
Subject classification (UKÄ)
- Biochemistry and Molecular Biology
Free keywords
- bioanalysis
- flow-ELISA
- immunoassay
- mycotoxin
- sequential injection
- zearalenone