Development of microformat imaging microplate and membrane immunoenzyme assays of the herbicide atrazine

O D Hendrickson; Ioana Wärnmark; R Sitdikov; A V Zherdev; B B Dzantiev; Bengt Danielsson

doi:10.1080/03067310500151144

Development of microformat imaging microplate and membrane immunoenzyme assays of the herbicide atrazine

O D Hendrickson, Ioana Wärnmark, R Sitdikov, A V Zherdev, B B Dzantiev, Bengt Danielsson

Pure and Applied Biochemistry

Research output: Contribution to journal › Article › peer-review

Abstract

A microformat imaging technique in combination with registration of chemiluminescence by a charge-coupled device, CCD camera, were applied for the detection of the herbicide atrazine by two immunoenzyme assay formats - microplate and membrane assays. For the microplate (ELISA) case the detection limit was 95 pg mL(-1), giving a 9.5-fold enhancement in comparison with a traditional colorimetric assay. The membrane dot blot assay allowed detection of up to 40 pg mL(-1) of atrazine (4.5-fold enhancement). Due to the application of polyelectrolyte carriers for rapid reactants separation the membrane assay may be realized in 15 min. CCD-based registration of signals assures a high reproducibility of measurements; the variation coefficient is in the range 1-7%. The effectiveness of the assays developed for controlling triazine herbicides in mineral and tap water has been demonstrated.

Original language	English
Pages (from-to)	905-915
Journal	International Journal of Environmental Analytical Chemistry
Volume	85
Issue number	12-13
DOIs	https://doi.org/10.1080/03067310500151144
Publication status	Published - 2005

Subject classification (UKÄ)

Biochemistry and Molecular Biology

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10.1080/03067310500151144

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title = "Development of microformat imaging microplate and membrane immunoenzyme assays of the herbicide atrazine",

abstract = "A microformat imaging technique in combination with registration of chemiluminescence by a charge-coupled device, CCD camera, were applied for the detection of the herbicide atrazine by two immunoenzyme assay formats - microplate and membrane assays. For the microplate (ELISA) case the detection limit was 95 pg mL(-1), giving a 9.5-fold enhancement in comparison with a traditional colorimetric assay. The membrane dot blot assay allowed detection of up to 40 pg mL(-1) of atrazine (4.5-fold enhancement). Due to the application of polyelectrolyte carriers for rapid reactants separation the membrane assay may be realized in 15 min. CCD-based registration of signals assures a high reproducibility of measurements; the variation coefficient is in the range 1-7%. The effectiveness of the assays developed for controlling triazine herbicides in mineral and tap water has been demonstrated.",

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AU - Hendrickson, O D

AU - Wärnmark, Ioana

AU - Sitdikov, R

AU - Zherdev, A V

AU - Dzantiev, B B

AU - Danielsson, Bengt

PY - 2005

Y1 - 2005

N2 - A microformat imaging technique in combination with registration of chemiluminescence by a charge-coupled device, CCD camera, were applied for the detection of the herbicide atrazine by two immunoenzyme assay formats - microplate and membrane assays. For the microplate (ELISA) case the detection limit was 95 pg mL(-1), giving a 9.5-fold enhancement in comparison with a traditional colorimetric assay. The membrane dot blot assay allowed detection of up to 40 pg mL(-1) of atrazine (4.5-fold enhancement). Due to the application of polyelectrolyte carriers for rapid reactants separation the membrane assay may be realized in 15 min. CCD-based registration of signals assures a high reproducibility of measurements; the variation coefficient is in the range 1-7%. The effectiveness of the assays developed for controlling triazine herbicides in mineral and tap water has been demonstrated.

AB - A microformat imaging technique in combination with registration of chemiluminescence by a charge-coupled device, CCD camera, were applied for the detection of the herbicide atrazine by two immunoenzyme assay formats - microplate and membrane assays. For the microplate (ELISA) case the detection limit was 95 pg mL(-1), giving a 9.5-fold enhancement in comparison with a traditional colorimetric assay. The membrane dot blot assay allowed detection of up to 40 pg mL(-1) of atrazine (4.5-fold enhancement). Due to the application of polyelectrolyte carriers for rapid reactants separation the membrane assay may be realized in 15 min. CCD-based registration of signals assures a high reproducibility of measurements; the variation coefficient is in the range 1-7%. The effectiveness of the assays developed for controlling triazine herbicides in mineral and tap water has been demonstrated.

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DO - 10.1080/03067310500151144

M3 - Article

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VL - 85

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EP - 915

JO - International Journal of Environmental Analytical Chemistry

JF - International Journal of Environmental Analytical Chemistry

IS - 12-13

ER -

Development of microformat imaging microplate and membrane immunoenzyme assays of the herbicide atrazine

Abstract

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