Differences in estimates of cisplatin-induced cell kill in vitro between colorimetric and cell count/colony assays

Eva Henriksson, Elisabeth Kjellén, Peter Wahlberg, Johan Wennerberg, Johan Kjellström

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30 Citations (SciVal)

Abstract

The aim of this study was to evaluate some bioassays that are different in principle: cell counting, colony forming assay, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT), sulforhodamine B (SRB), crystal violet, and alamarBlue, with respect to their ability to measure cisplatin-induced cell death of in vitro-cultivated squamous cell carcinoma of the head and neck (SCCHN). Cisplatin was applied in concentrations of 1.0, 5.0, 10.0, 50.0, and 100 mu M. The cells were incubated for 1 h, and the cell survival was measured 5 d after treatment. We found the colorimetric assays and cell counting to be comparable. The colony forming assay indicated a higher degree of cell kill compared with the other techniques. Measurement of cell survival after treatment with cisplatin can be done by use of any of the above tested assays. However, the majority of SCCHN cell lines available do not form colonies easily, or at all. Therefore, comparing the chemosensitivity between such cell lines is limited to alternative assays. In this respect, any of the tested colorimetric assays can be used. However, they seem to underestimate cell kill. Cell counting is also an alternative. This technique, however, is time consuming and operator dependent, as in the ease of manual counting, or relatively expensive when counting is performed electronically, compared with the colorimetric assays.
Original languageEnglish
Pages (from-to)320-323
JournalIn Vitro Cellular & Developmental Biology - Animal
Volume42
Issue number10
Publication statusPublished - 2006

Bibliographical note

The information about affiliations in this record was updated in December 2015.
The record was previously connected to the following departments: Otorhinolaryngology (Lund) (013044000), Oncology, MV (013035000), Reconstructive Surgery (013240300)

Subject classification (UKÄ)

  • Otorhinolaryngology
  • Cancer and Oncology

Keywords

  • crystal violet
  • alamarBlue
  • in vitro
  • MTT
  • SRB

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