Differential Effects of Ferulic Acid and p-Coumaric Acid on S Phase Distribution and Length of S Phase in the Human Colonic Cell Line Caco-2

Birgit Janicke; Gunilla Önning; S M Oredsson

doi:10.1021/jf050489l

Differential Effects of Ferulic Acid and p-Coumaric Acid on S Phase Distribution and Length of S Phase in the Human Colonic Cell Line Caco-2

Birgit Janicke, Gunilla Önning, S M Oredsson

Research output: Contribution to journal › Article › peer-review

Abstract

Ferulic acid (FA) and para-coumaric acid (p-CA) may mediate the protective effects of whole-grain cereals against colon cancer. Therefore, the effects of FA and p-CA on the metabolic activity, proliferation, cell cycle phase distribution, and kinetics of the colonic endothelial tumor cell line Caco-2 was studied. Both compounds at 1500 M decreased the number of cells to 43-75% of control after 2-3 days of treatment. Cell cycle phase distribution and cell cycle kinetics were determined by flow cytometric analysis after bromodeoxyuridine labeling. Each compound at 1500 M decreased the proportion of cells in the G1 phase and increased the proportion of cells in the S and G2 phases. Treatment with 1500 M FA significantly increased the length of the S phase, while p-CA did not. It was concluded that FA and p-CA inhibited cell proliferation by presumably affecting different cell cycle phases, and this warrants further investigations because this inhibition may be one explanation for the diet-related protection against cancer.

Original language	English
Pages (from-to)	6658-6665
Journal	Journal of Agricultural and Food Chemistry
Volume	53
Issue number	17
DOIs	https://doi.org/10.1021/jf050489l
Publication status	Published - 2005

Bibliographical note

The information about affiliations in this record was updated in December 2015.
The record was previously connected to the following departments: Pure and Applied Biochemistry (LTH) (011001005), Animal Physiology (Closed 2011) (011011000), Center for Chemistry and Chemical Engineering (011001000)

Subject classification (UKÄ)

Agricultural Science, Forestry and Fisheries

Free keywords

p-coumaric acid
Cell cycle
proliferation
cereals
ferulic acid

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1021/jf050489l

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title = "Differential Effects of Ferulic Acid and p-Coumaric Acid on S Phase Distribution and Length of S Phase in the Human Colonic Cell Line Caco-2",

abstract = "Ferulic acid (FA) and para-coumaric acid (p-CA) may mediate the protective effects of whole-grain cereals against colon cancer. Therefore, the effects of FA and p-CA on the metabolic activity, proliferation, cell cycle phase distribution, and kinetics of the colonic endothelial tumor cell line Caco-2 was studied. Both compounds at 1500 M decreased the number of cells to 43-75% of control after 2-3 days of treatment. Cell cycle phase distribution and cell cycle kinetics were determined by flow cytometric analysis after bromodeoxyuridine labeling. Each compound at 1500 M decreased the proportion of cells in the G1 phase and increased the proportion of cells in the S and G2 phases. Treatment with 1500 M FA significantly increased the length of the S phase, while p-CA did not. It was concluded that FA and p-CA inhibited cell proliferation by presumably affecting different cell cycle phases, and this warrants further investigations because this inhibition may be one explanation for the diet-related protection against cancer.",

keywords = "p-coumaric acid, Cell cycle, proliferation, cereals, ferulic acid",

author = "Birgit Janicke and Gunilla {\"O}nning and Oredsson, {S M}",

note = "The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Pure and Applied Biochemistry (LTH) (011001005), Animal Physiology (Closed 2011) (011011000), Center for Chemistry and Chemical Engineering (011001000)",

year = "2005",

doi = "10.1021/jf050489l",

language = "English",

volume = "53",

pages = "6658--6665",

journal = "Journal of Agricultural and Food Chemistry",

issn = "0021-8561",

publisher = "The American Chemical Society (ACS)",

number = "17",

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TY - JOUR

T1 - Differential Effects of Ferulic Acid and p-Coumaric Acid on S Phase Distribution and Length of S Phase in the Human Colonic Cell Line Caco-2

AU - Janicke, Birgit

AU - Önning, Gunilla

AU - Oredsson, S M

N1 - The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Pure and Applied Biochemistry (LTH) (011001005), Animal Physiology (Closed 2011) (011011000), Center for Chemistry and Chemical Engineering (011001000)

PY - 2005

Y1 - 2005

N2 - Ferulic acid (FA) and para-coumaric acid (p-CA) may mediate the protective effects of whole-grain cereals against colon cancer. Therefore, the effects of FA and p-CA on the metabolic activity, proliferation, cell cycle phase distribution, and kinetics of the colonic endothelial tumor cell line Caco-2 was studied. Both compounds at 1500 M decreased the number of cells to 43-75% of control after 2-3 days of treatment. Cell cycle phase distribution and cell cycle kinetics were determined by flow cytometric analysis after bromodeoxyuridine labeling. Each compound at 1500 M decreased the proportion of cells in the G1 phase and increased the proportion of cells in the S and G2 phases. Treatment with 1500 M FA significantly increased the length of the S phase, while p-CA did not. It was concluded that FA and p-CA inhibited cell proliferation by presumably affecting different cell cycle phases, and this warrants further investigations because this inhibition may be one explanation for the diet-related protection against cancer.

AB - Ferulic acid (FA) and para-coumaric acid (p-CA) may mediate the protective effects of whole-grain cereals against colon cancer. Therefore, the effects of FA and p-CA on the metabolic activity, proliferation, cell cycle phase distribution, and kinetics of the colonic endothelial tumor cell line Caco-2 was studied. Both compounds at 1500 M decreased the number of cells to 43-75% of control after 2-3 days of treatment. Cell cycle phase distribution and cell cycle kinetics were determined by flow cytometric analysis after bromodeoxyuridine labeling. Each compound at 1500 M decreased the proportion of cells in the G1 phase and increased the proportion of cells in the S and G2 phases. Treatment with 1500 M FA significantly increased the length of the S phase, while p-CA did not. It was concluded that FA and p-CA inhibited cell proliferation by presumably affecting different cell cycle phases, and this warrants further investigations because this inhibition may be one explanation for the diet-related protection against cancer.

KW - p-coumaric acid

KW - Cell cycle

KW - proliferation

KW - cereals

KW - ferulic acid

U2 - 10.1021/jf050489l

DO - 10.1021/jf050489l

M3 - Article

C2 - 16104781

SN - 0021-8561

VL - 53

SP - 6658

EP - 6665

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

IS - 17

ER -

Differential Effects of Ferulic Acid and p-Coumaric Acid on S Phase Distribution and Length of S Phase in the Human Colonic Cell Line Caco-2

Abstract

Bibliographical note

Subject classification (UKÄ)

Free keywords

UN SDGs

Access to Document

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Cite this