Differential effects of insulin-like growth factor-I on matrix and DNA synthesis in various regions and types of rabbit tendons

S. O. Abrahamsson, S. Lohmander

Research output: Contribution to journalArticlepeer-review

Abstract

Tendon healing and integration of tendon grafts may be site or donor specific. To determine if differences exist in sensitivity to growth factors that have the potential to influence tendon repair, we compared the effect of recombinant human insulin-like growth factor-1 on various types of tendon segments. The dose response effects on proteoglycan, collagen, noncollagen protein, and DNA synthesis were investigated in short-term explant cultures of intrasynovial intermediated and proximal segments of deep flexor tendons, extrasynovial segments of deep flexor tendons, and Achilles tendons of rabbits. The four different types of tendon segments cultured in media without recombinant human insulin-like growth factor-I synthesized similar amounts of each of the matrix components. Intrasynovial proximal segments synthesized 15 times less DNA than other tendon segments. Recombinant human insulin-like growth factor-1 stimulated matrix and DNA synthesis of all tendon segments in a dose-dependent manner in intervals from 10 to 1,000 ng/ml. The potency (LogED50) of the stimulation did not differ between the segments. The estimated maximal stimulation (E(max)) of proteoglycan synthesis was lower, in intrasynovial proximal segments as compared with that of the other types of segments. In contrast, the estimated maximal stimulation of DNA synthesis by recombinant human insulin-like growth factor- 1 was 6-fold higher than controls in all types of tendons. These findings demonstrate differences in mitotic capacity between anatomical regions of tendons during culture without recombinant human insulin-like growth factor- 1 and in matrix synthesis after stimulant with it.

Original languageEnglish
Pages (from-to)370-376
Number of pages7
JournalJournal of Orthopaedic Research
Volume14
Issue number3
DOIs
Publication statusPublished - 1996 May
Externally publishedYes

Subject classification (UKÄ)

  • Cell and Molecular Biology

Fingerprint

Dive into the research topics of 'Differential effects of insulin-like growth factor-I on matrix and DNA synthesis in various regions and types of rabbit tendons'. Together they form a unique fingerprint.

Cite this