Differential expression of chemokine receptors on human IgA plus and IgG plus B cells

C Johansson, I Ahlstedt, S Furubacka, E Johnsson, William Agace, M Quiding-Jarbrink

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Organ-specific lymphocyte homing is dependent on the expression of tissue-specific homing receptors and selected chemokine receptors. During the effector phase of an immune response, IgA and IgG antibody-secreting cells (ASC) are differently distributed in the body. Still, B cell expression of L-selectin and the mucosal homing receptor integrin alpha 4 beta 7 is not related to the isotype produced, but only to the site of antigen encounter. In this study, we examined if differences in chemokine responsiveness between IgA+ and IgG+ B cells could explain their different tissue localization. Circulating CD19+ B cells were isolated and their expression of IgA, IgG, and selected chemokine receptors was determined by flow cytometry. Few Ig+ cells expressed CCR2, CCR3, or CCR9, and there was no difference in the expression of these receptors between IgA+ and IgG+ cells. In contrast, CCR4, CCR5, and CXCR3 was expressed on significantly more IgG+ than IgA+ cells. The function of chemokine receptors on memory B cells and ASC was then tested in the transwell system. IgG+ memory cells migrated to a higher extent than IgA+ cells towards the CXCR3 ligand CXCL11/I-TAC, while there was only a small migration towards the CCR4 ligand CCL17/TARC and the CCR9 ligand CCL25/TECK. ASC migrated poorly to all chemokines tested. In conclusion, this study shows that IgG+ and IgA+ memory B cells have a differential expression of the Th1 associated chemokine receptor CXCR3, as well as of CCR4 and CCR5. In contrast, none of the studied chemokine receptors was preferentially expressed by IgA+ cells.
    Original languageEnglish
    Pages (from-to)279-287
    JournalClinical and Experimental Immunology
    Volume141
    Issue number2
    DOIs
    Publication statusPublished - 2005

    Subject classification (UKÄ)

    • Immunology in the medical area

    Free keywords

    • lymphocyte trafficking
    • chemokine receptor
    • B cell
    • human
    • antibody-secreting cell

    Fingerprint

    Dive into the research topics of 'Differential expression of chemokine receptors on human IgA plus and IgG plus B cells'. Together they form a unique fingerprint.

    Cite this