Diffusion studies in gels using holographic laser interferometry

Charlotte Mattisson

Research output: ThesisDoctoral Thesis (monograph)

Abstract

This thesis is an investigation of the potential of holographic laser interferometry for the study of diffusion of both small solutes and macromolecules. An investigation on the importance of errors due to light deflection in refarctive index gradients showed that the magnitude of the refractive index gradients were negligible for the system investigated. The holographic interferometry technique requires a high stability to study diffusion of macromolecules. This was achieved by a proper design and hence the diffusion coefficients for BSA, lysozyme and pullulan in agarose gels were succesfully obtained. The effect of solvent as well as the BSA and agarose concentration on the BSA diffusion coefficient and partition coefficient was investigated. In addition, the effect of the pullulan molar weight on the pullulan diffusion and partition coefficient in agarose gel was studied. The effect of pH and ionic strength on the lysozyme diffusion in agarose gel was studied. The experimental data was evaluated using a general transport model based on chemical potential gradients instead of concentration gradients. The chemical potential was estimated by using the Poisson-Boltzmann equation.
Original languageEnglish
QualificationDoctor
Awarding Institution
  • Division of Chemical Engineering
Supervisors/Advisors
  • Axelsson, Anders, Supervisor
  • Zacchi, Guido, Supervisor
Award date1999 Jun 11
Publisher
Publication statusPublished - 1999

Bibliographical note

Defence details

Date: 1999-06-11
Time: 10:15
Place: Lund University, Chemical Centre, lecture hall K:C

External reviewer(s)

Name: Cussler, E.L.
Title: Professor
Affiliation: University of Minnesota, Minneapolis, USA

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Subject classification (UKÄ)

  • Chemical Engineering

Free keywords

  • chemical potential
  • ionic strenght
  • pH
  • cubic phase
  • partition coefficient
  • holographic laser interferometry
  • holography
  • pullulan
  • lysozyme
  • BSA
  • macromolecules
  • diffusion
  • gel
  • Poisson-Boltzmann
  • Chemical technology and engineering
  • Kemiteknik och kemisk teknologi

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