Direct Detection Of Single-Nucleotide Polymorphisms In Bacterial DNA By SNPtrap

Hugo Gronlund, Birgitte Moen, Jeffrey Hoorfar, Peter Rådström, Burkhard Malorny, Knut Rudi

Research output: Contribution to journalArticlepeer-review

Abstract

A major challenge with single-nucleotide polymorphism (SNP) fingerprinting of bacteria and higher organisms is the combination of genome-wide screenings with the potential of multiplexing and accurate SNP detection. Single-nucleotide extension by the minisequencing principle represents a technology that both is highly accurate and enables multiplexing. A current bottleneck for direct genome analyses by minisequencing, however, is the sensitivity, since minisequencing relies on linear signal amplification. Here, we present SNPtrap, which is a novel approach that combines the specificity and possibility of multiplexing by minisequencing with the sensitivity obtained by logarithmic signal amplification by polymerase chain reaction (PCR). We show a SNPtrap proof of principle in a model system for two polymorphic SNP sites in the Salmonella tetrathionate reductase gene (ttrC).
Original languageEnglish
Pages (from-to)166-174
JournalPreparative Biochemistry & Biotechnology
Volume41
Issue number2
DOIs
Publication statusPublished - 2011

Subject classification (UKÄ)

  • Industrial Biotechnology

Keywords

  • multiplex
  • Salmonella
  • SNP

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