Direct Reprogramming of Human Fetal- and Stem Cell-Derived Glial Progenitor Cells into Midbrain Dopaminergic Neurons

Sara Nolbrant, Jessica Giacomoni, Deirdre Hoban, Andreas Bruzelius, Marcella Birtele, Devin Chandler-Militello, Maria Pereira, Daniella Rylander Ottosson, Steven A. Goldman, Malin Parmar

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)
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Abstract

Human glial progenitor cells (hGPCs) are promising cellular substrates to explore for the in situ production of new neurons for brain repair. Proof of concept for direct neuronal reprogramming of glial progenitors has been obtained in mouse models in vivo, but conversion using human cells has not yet been demonstrated. Such studies have been difficult to perform since hGPCs are born late during human fetal development, with limited accessibility for in vitro culture. In this study, we show proof of concept of hGPC conversion using fetal cells and also establish a renewable and reproducible stem cell-based hGPC system for direct neural conversion in vitro. Using this system, we have identified optimal combinations of fate determinants for the efficient dopaminergic (DA) conversion of hGPCs, thereby yielding a therapeutically relevant cell type that selectively degenerates in Parkinson's disease. The induced DA neurons show a progressive, subtype-specific phenotypic maturation and acquire functional electrophysiological properties indicative of DA phenotype.
Original languageEnglish
Pages (from-to)869-882
JournalStem Cell Reports
Volume15
Issue number4
DOIs
Publication statusPublished - 2020

Bibliographical note

Copyright © 2020 The Authors. Published by Elsevier Inc. All rights reserved.

Subject classification (UKÄ)

  • Neurosciences

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