Disposable Electrodes for Capacitive Immunosensor

Kosin Teeparuksapun, Proespichaya Kanatharana, Warakorn Limbut, Chongdee Thammakhet, Punnee Asawatreratanakul, Bo Mattiasson, Booncharoen Wongkittisuksa, Chusak Limsakul, Panote Thavarungkul

Research output: Contribution to journalArticlepeer-review

22 Citations (SciVal)

Abstract

Disposable electrodes were fabricated by coating chromium (5 nm) and gold (200 nm) on glass strips (5.0 mm x 25.4 mm) and used in a label-free immunosensor. Human Serum albumin (HSA) and its antigen (anti-HSA) were used as a model system. Electropolymerization of o-phenylenediamine was used for the immobilization of anti-HSA by covalent binding. A linear relationship was obtained in the range from 1.0 x 10-(14) to 1.0 x 10-(19) M with a limit of detection of 8.0 x 10(-15) M. Each modified electrode can be reused up to 30 times. The developed system was applied for human serum samples and compared to Albumin BCG method.
Original languageEnglish
Pages (from-to)1066-1074
JournalElectroanalysis
Volume21
Issue number9
DOIs
Publication statusPublished - 2009

Subject classification (UKÄ)

  • Industrial Biotechnology

Keywords

  • Capacitive immunosensor
  • o-Phenylenediamine
  • Disposable electrode
  • Thermal evaporation
  • Human serum albumin

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