Distinct myeloid progenitor-differentiation pathways identified through single-cell RNA sequencing

Roy Drissen, Natalija Buza-Vidas, Petter Woll, Supat Thongjuea, Adriana Gambardella, Alice Giustacchini, Elena Mancini, Alya Zriwil, Michael Lutteropp, Amit Grover, Adam Mead, Ewa Sitnicka, Sten Eirik W Jacobsen, Claus Nerlov

Research output: Contribution to journalArticlepeer-review


According to current models of hematopoiesis, lymphoid-primed multi-potent progenitors (LMPPs) (Lin(-)Sca-1(+)c-Kit(+)CD34(+)Flt3(hi)) and common myeloid progenitors (CMPs) (Lin(-)Sca-1(+)c-Kit(+)CD34(+)CD41(hi)) establish an early branch point for separate lineage-commitment pathways from hematopoietic stem cells, with the notable exception that both pathways are proposed to generate all myeloid innate immune cell types through the same myeloid-restricted pre-granulocyte-macrophage progenitor (pre-GM) (Lin(-)Sca-1(-)c-Kit(+)CD41(-)FcγRII/III(-)CD150(-)CD105(-)). By single-cell transcriptome profiling of pre-GMs, we identified distinct myeloid differentiation pathways: a pathway expressing the gene encoding the transcription factor GATA-1 generated mast cells, eosinophils, megakaryocytes and erythroid cells, and a pathway lacking expression of that gene generated monocytes, neutrophils and lymphocytes. These results identify an early hematopoietic-lineage bifurcation that separates the myeloid lineages before their segregation from other hematopoietic-lineage potential.

Original languageEnglish
Pages (from-to)666-676
JournalNature Immunology
Issue number6
Early online date2016 Apr 4
Publication statusPublished - 2016 Jun

Subject classification (UKÄ)

  • Immunology


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