Diversity of human papillomaviruses in skin lesions.

Johanna Ekström; Laila Sara Arroyo Mühr; Davit Bzhalava; Anna Söderlund Strand; Emilie Hultin; Peter Nordin; Bo Stenquist; John Paoli; Ola Forslund; Joakim Dillner

doi:10.1016/j.virol.2013.09.010

Diversity of human papillomaviruses in skin lesions.

Johanna Ekström, Laila Sara Arroyo Mühr, Davit Bzhalava, Anna Söderlund Strand, Emilie Hultin, Peter Nordin, Bo Stenquist, John Paoli, Ola Forslund, Joakim Dillner

Research output: Contribution to journal › Article › peer-review

Abstract

Pools of frozen biopsies from patients with squamous cell carcinoma (SCC) (n=29) actinic keratosis (AK) (n=31), keratoacanthoma (n=91) and swab samples from 84 SCCs and 91 AKs were analysed with an extended HPV general primer PCR and high-throughput sequencing of amplimers. We found 273 different HPV isolates (87 known HPV types, 139 previously known HPV sequences (putative types) and 47 sequences from novel putative HPV types). Among the new sequences, five clustered in genus Betapapillomavirus and 42 in genus Gammapapillomavirus. Resequencing of the three pools between 21 to 70 times resulted in the detection of 283 different known or putative HPV types, with 156 different sequences found in only one of the pools. Type-specific PCRs for 37 putative types from an additional 296 patients found only two of these putative types. In conclusion, skin lesions contain a large diversity of HPV types, but most appeared to be rare infections.

Original language	English
Pages (from-to)	300-311
Journal	Virology
Volume	447
Issue number	1-2
DOIs	https://doi.org/10.1016/j.virol.2013.09.010
Publication status	Published - 2013

Subject classification (UKÄ)

Microbiology in the Medical Area

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10.1016/j.virol.2013.09.010

http://www.ncbi.nlm.nih.gov/pubmed/24210127?dopt=Abstract

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@article{4eb82c77c1e24962bec9203d46a763a2,

title = "Diversity of human papillomaviruses in skin lesions.",

abstract = "Pools of frozen biopsies from patients with squamous cell carcinoma (SCC) (n=29) actinic keratosis (AK) (n=31), keratoacanthoma (n=91) and swab samples from 84 SCCs and 91 AKs were analysed with an extended HPV general primer PCR and high-throughput sequencing of amplimers. We found 273 different HPV isolates (87 known HPV types, 139 previously known HPV sequences (putative types) and 47 sequences from novel putative HPV types). Among the new sequences, five clustered in genus Betapapillomavirus and 42 in genus Gammapapillomavirus. Resequencing of the three pools between 21 to 70 times resulted in the detection of 283 different known or putative HPV types, with 156 different sequences found in only one of the pools. Type-specific PCRs for 37 putative types from an additional 296 patients found only two of these putative types. In conclusion, skin lesions contain a large diversity of HPV types, but most appeared to be rare infections.",

author = "Johanna Ekstr{\"o}m and M{\"u}hr, {Laila Sara Arroyo} and Davit Bzhalava and {S{\"o}derlund Strand}, Anna and Emilie Hultin and Peter Nordin and Bo Stenquist and John Paoli and Ola Forslund and Joakim Dillner",

year = "2013",

doi = "10.1016/j.virol.2013.09.010",

language = "English",

volume = "447",

pages = "300--311",

journal = "Virology",

issn = "1096-0341",

publisher = "Elsevier",

number = "1-2",

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TY - JOUR

T1 - Diversity of human papillomaviruses in skin lesions.

AU - Ekström, Johanna

AU - Mühr, Laila Sara Arroyo

AU - Bzhalava, Davit

AU - Söderlund Strand, Anna

AU - Hultin, Emilie

AU - Nordin, Peter

AU - Stenquist, Bo

AU - Paoli, John

AU - Forslund, Ola

AU - Dillner, Joakim

PY - 2013

Y1 - 2013

N2 - Pools of frozen biopsies from patients with squamous cell carcinoma (SCC) (n=29) actinic keratosis (AK) (n=31), keratoacanthoma (n=91) and swab samples from 84 SCCs and 91 AKs were analysed with an extended HPV general primer PCR and high-throughput sequencing of amplimers. We found 273 different HPV isolates (87 known HPV types, 139 previously known HPV sequences (putative types) and 47 sequences from novel putative HPV types). Among the new sequences, five clustered in genus Betapapillomavirus and 42 in genus Gammapapillomavirus. Resequencing of the three pools between 21 to 70 times resulted in the detection of 283 different known or putative HPV types, with 156 different sequences found in only one of the pools. Type-specific PCRs for 37 putative types from an additional 296 patients found only two of these putative types. In conclusion, skin lesions contain a large diversity of HPV types, but most appeared to be rare infections.

AB - Pools of frozen biopsies from patients with squamous cell carcinoma (SCC) (n=29) actinic keratosis (AK) (n=31), keratoacanthoma (n=91) and swab samples from 84 SCCs and 91 AKs were analysed with an extended HPV general primer PCR and high-throughput sequencing of amplimers. We found 273 different HPV isolates (87 known HPV types, 139 previously known HPV sequences (putative types) and 47 sequences from novel putative HPV types). Among the new sequences, five clustered in genus Betapapillomavirus and 42 in genus Gammapapillomavirus. Resequencing of the three pools between 21 to 70 times resulted in the detection of 283 different known or putative HPV types, with 156 different sequences found in only one of the pools. Type-specific PCRs for 37 putative types from an additional 296 patients found only two of these putative types. In conclusion, skin lesions contain a large diversity of HPV types, but most appeared to be rare infections.

U2 - 10.1016/j.virol.2013.09.010

DO - 10.1016/j.virol.2013.09.010

M3 - Article

C2 - 24210127

SN - 1096-0341

VL - 447

SP - 300

EP - 311

JO - Virology

JF - Virology

IS - 1-2

ER -

Diversity of human papillomaviruses in skin lesions.

Abstract

Subject classification (UKÄ)

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