DNA ejection from bacteriophage: Towards a general behavior for osmotic-suppression experiments

M. Castelnovo; Alex Evilevitch

doi:10.1140/epje/i2007-10205-5

DNA ejection from bacteriophage: Towards a general behavior for osmotic-suppression experiments

M. Castelnovo, Alex Evilevitch

Biochemistry and Structural Biology

Research output: Contribution to journal › Article › peer-review

Abstract

We present in this work in vitro measurements of the force ejecting DNA from two distinct bacteriophages (T5 and lambda using the osmotic-suppression technique. Our data are analyzed by revisiting the current theories of DNA packaging in spherical capsids. In particular we show that a simplified analytical model based on bending considerations only is able to account quantitatively for the experimental findings. Physical and biological consequences are discussed.

Original language	English
Pages (from-to)	9-18
Journal	European Physical Journal E. Soft Matter
Volume	24
Issue number	1
DOIs	https://doi.org/10.1140/epje/i2007-10205-5
Publication status	Published - 2007

Subject classification (UKÄ)

Biological Sciences

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10.1140/epje/i2007-10205-5

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title = "DNA ejection from bacteriophage: Towards a general behavior for osmotic-suppression experiments",

abstract = "We present in this work in vitro measurements of the force ejecting DNA from two distinct bacteriophages (T5 and lambda using the osmotic-suppression technique. Our data are analyzed by revisiting the current theories of DNA packaging in spherical capsids. In particular we show that a simplified analytical model based on bending considerations only is able to account quantitatively for the experimental findings. Physical and biological consequences are discussed.",

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AU - Evilevitch, Alex

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AB - We present in this work in vitro measurements of the force ejecting DNA from two distinct bacteriophages (T5 and lambda using the osmotic-suppression technique. Our data are analyzed by revisiting the current theories of DNA packaging in spherical capsids. In particular we show that a simplified analytical model based on bending considerations only is able to account quantitatively for the experimental findings. Physical and biological consequences are discussed.

U2 - 10.1140/epje/i2007-10205-5

DO - 10.1140/epje/i2007-10205-5

M3 - Article

SN - 1292-8941

VL - 24

SP - 9

EP - 18

JO - European Physical Journal E. Soft Matter

JF - European Physical Journal E. Soft Matter

IS - 1

ER -

DNA ejection from bacteriophage: Towards a general behavior for osmotic-suppression experiments

Abstract

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