Dried-Reswollen Immobilized Biocatalysts for Detoxification of Organophosphorous Compounds in the Flow Systems

Elena N. Efremenko, Ilya V. Lyagin, Fatima Plieva, Igor Galaev, Bo Mattiasson

Research output: Contribution to journalArticlepeer-review

5 Citations (SciVal)

Abstract

New immobilized biocatalysts based on polypeptides containing N- or C-terminal polyhistidine sequences and possessing organophosphorus hydrolase activity were investigated for detoxification of organophosphorous neurotoxic compounds in the flow systems. The biocatalysts were revealed to have a high catalytic activity within wide pH and temperature ranges 7.5-12.5 A degrees C and 15-65 A degrees C, respectively. The immobilized biocatalysts can be dried and reswollen before use with 92-93% catalytic activity remaining after drying and rehydration procedures. The half-lives of the biocatalysts under wet and dry storage conditions were 420 and 540 days, respectively.
Original languageEnglish
Pages (from-to)251-260
JournalApplied Biochemistry and Biotechnology
Volume159
Issue number1
DOIs
Publication statusPublished - 2009

Subject classification (UKÄ)

  • Biochemistry and Molecular Biology

Keywords

  • immobilized biocatalyst
  • Dried/reswollen
  • Organophosphorus hydrolase
  • Polyhistidine sequence
  • Organophosphorous compound

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