TY - JOUR
T1 - Dual-focus coherent anti-Stokes Raman scattering microscopy using a compact two-beam fiber laser source
AU - Legesse, Fisseha Bekele
AU - Meyer, Tobias
AU - Heuke, Sandro
AU - Gottschall, Thomas
AU - Pascher, Torbjörn
AU - Limpert, Jens
AU - Tünnermann, Andreas
AU - Schmitt, Michael
AU - Popp, Jürgen
PY - 2017/1/15
Y1 - 2017/1/15
N2 - We have developed a dual-focus coherent anti-Stokes Raman scattering (CARS) microscope based on a dual output, compact fiber laser source. The underlying concepts of time-multiplexed, two-beam scanning and demultiplexed detection that we already employed for second-harmonic generation are here naturally extended for CARS microscopy. The layout of a robust, all-fiber laser source was reconfigured to provide two outputs, each containing the two colors necessary for the CARS process. The utilization of the design for simultaneously imaging two laterally or axially separated fields of view and, thus, inherently speeding up the image acquisition process, is demonstrated on human artery tissue samples.
AB - We have developed a dual-focus coherent anti-Stokes Raman scattering (CARS) microscope based on a dual output, compact fiber laser source. The underlying concepts of time-multiplexed, two-beam scanning and demultiplexed detection that we already employed for second-harmonic generation are here naturally extended for CARS microscopy. The layout of a robust, all-fiber laser source was reconfigured to provide two outputs, each containing the two colors necessary for the CARS process. The utilization of the design for simultaneously imaging two laterally or axially separated fields of view and, thus, inherently speeding up the image acquisition process, is demonstrated on human artery tissue samples.
UR - http://www.scopus.com/inward/record.url?scp=85009476784&partnerID=8YFLogxK
U2 - 10.1364/OL.42.000183
DO - 10.1364/OL.42.000183
M3 - Article
C2 - 28081075
AN - SCOPUS:85009476784
SN - 0146-9592
VL - 42
SP - 183
EP - 186
JO - Optics Letters
JF - Optics Letters
IS - 2
ER -