Abstract
Introduction: Mesenchymal stem cells (MSCs) were proposed as a critical therapeutic candidate in diabetic nephropathy (DN). Renal stem cells as a source for repairing are controversial. The purpose of the present study was to evaluate the effect of kidney rat stem cells on DN.
Materials and methods: After separation of renal stem cells from rat kidney, the surface Stem cell markers were assessed by flow cytometry analysis. To establish the diabetic nephropathy rat model STZ(60mg/kg) was used. The cells were injected to experimental groups via tail vein (2×106cells/rat). Biochemical and histological parameters were evaluated in order to determining the impact of stem cells on kidney structure. Phosphorylation of Smad2/3 two weeks after induction of early diabetic nephropathy was evaluated by using standard western blotting.
Result: The cells significantly reduced blood nitrogen (BUN), serum creatinine (Scr) and 24 urinary proteins. The phosphorylation of smad2 and smad3 significantly down-regulated. PAS staining showed in the presence of adult kidney stem cells histopathological changes were improved.
Conclusion: Adult kidney stem cells may be a candidate for treatment of early DN to improve the kidney function and regenerating kidney tissues in DN rats.
Materials and methods: After separation of renal stem cells from rat kidney, the surface Stem cell markers were assessed by flow cytometry analysis. To establish the diabetic nephropathy rat model STZ(60mg/kg) was used. The cells were injected to experimental groups via tail vein (2×106cells/rat). Biochemical and histological parameters were evaluated in order to determining the impact of stem cells on kidney structure. Phosphorylation of Smad2/3 two weeks after induction of early diabetic nephropathy was evaluated by using standard western blotting.
Result: The cells significantly reduced blood nitrogen (BUN), serum creatinine (Scr) and 24 urinary proteins. The phosphorylation of smad2 and smad3 significantly down-regulated. PAS staining showed in the presence of adult kidney stem cells histopathological changes were improved.
Conclusion: Adult kidney stem cells may be a candidate for treatment of early DN to improve the kidney function and regenerating kidney tissues in DN rats.
| Original language | English |
|---|---|
| Pages | ID297 |
| Publication status | Accepted/In press - 2021 |
| Event | 6th world congress of the Tissue Engineering and Regenerative Medicine International Society (TERMIS2021) - Maastricht, Netherlands Duration: 2021 Nov 15 → 2021 Nov 19 https://termis.org/WC2021 |
Conference
| Conference | 6th world congress of the Tissue Engineering and Regenerative Medicine International Society (TERMIS2021) |
|---|---|
| Country/Territory | Netherlands |
| City | Maastricht |
| Period | 2021/11/15 → 2021/11/19 |
| Internet address |
Subject classification (UKÄ)
- Physiology and Anatomy
- Endocrinology and Diabetes
Free keywords
- Stem cell therapy
- smad signaling
- Diabetic nephropathy
- kidney stem cells
