Effects of acid concentration and solvent choice on enzymatic acrylation by Candida antarctica lipase B

Mathias Nordblad, Patrick Adlercreutz

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Lipase-mediated acrylation is an attractive alternative to more traditional chemical processes, since it provides specific catalysis under mild conditions. A detailed study of the effects of solvent choice and substrate concentrations on the acrylation of octanol by Candida antarctica lipase B (Novozyme(R) 435) is presented. Acrylic acid was found to have a pronounced inhibitory effect. Partial neutralisation of the acid substrate by addition of an organo-soluble base markedly altered the activity profile, indicating the inhibitory mechanism to be related to acid-base interactions. The concentration of acrylic acid to be employed was found to be important in the choice of an appropriate solvent. At low acrylic acid concentrations, the highest rates and conversions were obtained using hydrophobic solvents, whereas at higher acrylic acid concentrations more polar solvents were advantageous.
    Original languageEnglish
    Pages (from-to)127-133
    JournalJournal of Biotechnology
    Volume133
    Issue number1
    DOIs
    Publication statusPublished - 2008

    Subject classification (UKÄ)

    • Industrial Biotechnology

    Free keywords

    • protonation effects
    • acrylate synthesis
    • lipase
    • acrylic acid

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