Effects of dextran-linked chloromercuribenzoic acid on insulin release from microdissected pancreatic islets

Stig Åkerstrom; Bo Hellman; Åker Lernmark; Bernt Lindberg; Monica Söderberg; Inge Bert Täljedal

doi:10.1016/0304-4165(76)90261-0

Effects of dextran-linked chloromercuribenzoic acid on insulin release from microdissected pancreatic islets

Stig Åkerstrom, Bo Hellman, Åker Lernmark, Bernt Lindberg, Monica Söderberg, Inge Bert Täljedal

Umeå University

Research output: Contribution to journal › Article › peer-review

Abstract

Insulin release in response to dextran-linked p-chloromercuribenzoic acid was studied in microdissected pancreatic islets of non-inbred ob/ob-mice. No contamination of the dextran-linked mercurial with free chloromercuribenzoic acid was detected before or after the incubation with islets. In comparison with free mercurial, of the same thiol-blocking activity, the dextran-linked compound had a weak insulin-releasing action with a different dose vs. response relationship. The dextran-linked mercurial had no demonstrable effect on the islet content of cyclic AMP. The results support the hypothesis that free organic mercurials mainly stimulate insulin release by blocking thiol groups that are embedded within the β-cell plasma membranes beneath their surfaces.

Original language	English
Pages (from-to)	96-105
Number of pages	10
Journal	BBA - General Subjects
Volume	451
Issue number	1
DOIs	https://doi.org/10.1016/0304-4165(76)90261-0
Publication status	Published - 1976 Nov 18
Externally published	Yes

Subject classification (UKÄ)

Endocrinology and Diabetes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/0304-4165(76)90261-0

Cite this

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abstract = "Insulin release in response to dextran-linked p-chloromercuribenzoic acid was studied in microdissected pancreatic islets of non-inbred ob/ob-mice. No contamination of the dextran-linked mercurial with free chloromercuribenzoic acid was detected before or after the incubation with islets. In comparison with free mercurial, of the same thiol-blocking activity, the dextran-linked compound had a weak insulin-releasing action with a different dose vs. response relationship. The dextran-linked mercurial had no demonstrable effect on the islet content of cyclic AMP. The results support the hypothesis that free organic mercurials mainly stimulate insulin release by blocking thiol groups that are embedded within the β-cell plasma membranes beneath their surfaces.",

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AU - Åkerstrom, Stig

AU - Hellman, Bo

AU - Lernmark, Åker

AU - Lindberg, Bernt

AU - Söderberg, Monica

AU - Täljedal, Inge Bert

PY - 1976/11/18

Y1 - 1976/11/18

N2 - Insulin release in response to dextran-linked p-chloromercuribenzoic acid was studied in microdissected pancreatic islets of non-inbred ob/ob-mice. No contamination of the dextran-linked mercurial with free chloromercuribenzoic acid was detected before or after the incubation with islets. In comparison with free mercurial, of the same thiol-blocking activity, the dextran-linked compound had a weak insulin-releasing action with a different dose vs. response relationship. The dextran-linked mercurial had no demonstrable effect on the islet content of cyclic AMP. The results support the hypothesis that free organic mercurials mainly stimulate insulin release by blocking thiol groups that are embedded within the β-cell plasma membranes beneath their surfaces.

AB - Insulin release in response to dextran-linked p-chloromercuribenzoic acid was studied in microdissected pancreatic islets of non-inbred ob/ob-mice. No contamination of the dextran-linked mercurial with free chloromercuribenzoic acid was detected before or after the incubation with islets. In comparison with free mercurial, of the same thiol-blocking activity, the dextran-linked compound had a weak insulin-releasing action with a different dose vs. response relationship. The dextran-linked mercurial had no demonstrable effect on the islet content of cyclic AMP. The results support the hypothesis that free organic mercurials mainly stimulate insulin release by blocking thiol groups that are embedded within the β-cell plasma membranes beneath their surfaces.

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Effects of dextran-linked chloromercuribenzoic acid on insulin release from microdissected pancreatic islets

Abstract

Subject classification (UKÄ)

UN SDGs

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