Effects of ethanol on muscarinic receptor-stimulated c-fos expression in human neuroblastoma cells

W Q Ding, Christer Larsson, Per Simonsson, Christer Alling

Research output: Contribution to journalArticlepeer-review

Abstract

The effect of ethanol exposure on muscarinic receptor-stimulated expression of c-fos was investigated in SH-SY5Y cells. Four days of ethanol exposure enhanced carbachol-stimulated c-fos mRNA expression, analyzed with Northern blot, and Fos/AP-1 binding activity, measured with gel mobility super shift assay. Pre-incubation with muscarinic antagonists or the protein kinase C inhibitor GF109203X demonstrated that, in both control and ethanol-treated cells, carbachol-induced c-fos expression was mediated via muscarinic M1 receptors and to a large extent through protein kinase C. However, phorbol ester-induced c-fos expression was unaffected in ethanol-treated cells. Acute exposure to ethanol caused a suppression of both carbachol- and phorbol ester-stimulated c-fos expression. These results demonstrate that muscarinic receptor-stimulated gene expression is sensitive to both acute and long-term ethanol exposure.
Original languageEnglish
Pages (from-to)77-84
JournalBrain Research. Molecular Brain Research
Volume46
Issue number1-2
DOIs
Publication statusPublished - 1997

Subject classification (UKÄ)

  • Medicinal Chemistry
  • Clinical Medicine
  • Cancer and Oncology
  • Pharmacology and Toxicology

Keywords

  • Muscarinic acetylcholine receptor
  • Ethanol
  • c-fos expression
  • Protein kinase C
  • Neuroblastoma SH-SY5Y cell

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