Effects of inhibition of glycation and oxidative stress on the development of diabetic nephropathy in rats.

Carl David Agardh, Unne Stenram, Ole Torffvit, Elisabet Agardh

Research output: Contribution to journalArticlepeer-review

Abstract

We investigated whether aminoguanidine (AG), an inhibitor of advanced glycated end product formation, or probucol (PB), a free radical scavenger, could influence signs of glomerular and distal tubular function and morphological changes in kidneys of male Wistar rats after 6 months of streptozocin (STZ)-induced diabetes. Diabetic rats had a higher kidney weight/body weight ratio (P<.001), but neither AG nor PB influenced the increased ratio. Diabetes caused an increased urinary albumin excretion (P<.05), which was normalized by AG, but further exaggerated by PB (P<.001). Diabetes also caused an increase in the urinary excretion of Tamm–Horsfall protein (P<.001). Both AG and PB attenuated this increase (P<.05 for both). A few glomeruli displayed focal thickening of varying degrees. Silver staining disclosed the glomerulopathy to be intercapillary glomerulosclerosis. Rats on PB-enriched diet displayed less pronounced changes than untreated rats (P<.01), while AG had no effect. The results suggest that oxidative stress could be involved in the development of diabetic nephropathy.
Original languageEnglish
Pages (from-to)395-400
JournalJournal of Diabetes and its Complications
Volume16
Issue number6
DOIs
Publication statusPublished - 2002

Bibliographical note

The information about affiliations in this record was updated in December 2015.
The record was previously connected to the following departments: Medicine (Lund) (013230025), Ophthalmology (013242810), Pathology, (Lund) (013030000), Endocrinology (013241500)

Subject classification (UKÄ)

  • Endocrinology and Diabetes

Free keywords

  • Free Radical Scavengers: pharmacology
  • Diabetic Nephropathies: physiopathology
  • Disease Progression
  • Experimental: physiopathology
  • Probucol: pharmacology
  • Rats
  • Animal
  • Diabetes Mellitus
  • Non-U.S. Gov't
  • Distal: physiopathology
  • Male
  • Oxidative Stress: physiology
  • Support
  • Wistar
  • Kidney Tubules
  • Distal: pathology
  • Distal: drug effects
  • Kidney Glomerulus: physiopathology
  • Kidney Glomerulus: pathology
  • Kidney Glomerulus: drug effects
  • Guanidines: pharmacology
  • Advanced: antagonists & inhibitors
  • Glycosylation End Products
  • Glycosylation

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