Electrochemical wiring of the Gram-positive bacterium Enterococcus faecalis with osmium redox polymer modified electrodes

Galina Pankratova; Kamrul Hasan; Dónal Leech; Lars Hederstedt; Lo Gorton

doi:10.1016/j.elecom.2016.12.010

Electrochemical wiring of the Gram-positive bacterium Enterococcus faecalis with osmium redox polymer modified electrodes

Galina Pankratova, Kamrul Hasan, Dónal Leech, Lars Hederstedt, Lo Gorton

Biochemistry and Structural Biology

National University of Ireland Galway

Research output: Contribution to journal › Article › peer-review

Abstract

The Gram-positive bacterium Enterococcus faecalis was investigated to test its ability to electrochemically communicate with electrodes. E. faecalis cells were successfully “wired” to gold electrodes through [Os(2,2′-bipyridine)₂-poly(N-vinylimidazole)₁₀Cl]^2 +/+ redox polymer. Maximum current density achieved in response to 10 mM D-glucose was 18 ± 1 μA cm^− 2. Control experiments without redox polymer showed no current response. This demonstrates the efficiency of the osmium-based mediator in electron transfer between the cells having a thick cell wall and the electrode. The findings have significant importance in microbial electrochemistry for further understanding of microbe-electrode interactions.

Original language	English
Pages (from-to)	56-59
Number of pages	4
Journal	Electrochemistry Communications
Volume	75
DOIs	https://doi.org/10.1016/j.elecom.2016.12.010
Publication status	Published - 2017 Feb 1

Subject classification (UKÄ)

Biological Sciences
Microbiology

Free keywords

Electrochemical communication
Enterococcus faecalis
Gram-positive bacterium
Osmium redox polymer

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10.1016/j.elecom.2016.12.010

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title = "Electrochemical wiring of the Gram-positive bacterium Enterococcus faecalis with osmium redox polymer modified electrodes",

abstract = "The Gram-positive bacterium Enterococcus faecalis was investigated to test its ability to electrochemically communicate with electrodes. E. faecalis cells were successfully “wired” to gold electrodes through [Os(2,2′-bipyridine)2-poly(N-vinylimidazole)10Cl]2 +/+ redox polymer. Maximum current density achieved in response to 10 mM D-glucose was 18 ± 1 μA cm− 2. Control experiments without redox polymer showed no current response. This demonstrates the efficiency of the osmium-based mediator in electron transfer between the cells having a thick cell wall and the electrode. The findings have significant importance in microbial electrochemistry for further understanding of microbe-electrode interactions.",

keywords = "Electrochemical communication, Enterococcus faecalis, Gram-positive bacterium, Osmium redox polymer",

author = "Galina Pankratova and Kamrul Hasan and D{\'o}nal Leech and Lars Hederstedt and Lo Gorton",

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doi = "10.1016/j.elecom.2016.12.010",

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T1 - Electrochemical wiring of the Gram-positive bacterium Enterococcus faecalis with osmium redox polymer modified electrodes

AU - Pankratova, Galina

AU - Hasan, Kamrul

AU - Leech, Dónal

AU - Hederstedt, Lars

AU - Gorton, Lo

PY - 2017/2/1

Y1 - 2017/2/1

N2 - The Gram-positive bacterium Enterococcus faecalis was investigated to test its ability to electrochemically communicate with electrodes. E. faecalis cells were successfully “wired” to gold electrodes through [Os(2,2′-bipyridine)2-poly(N-vinylimidazole)10Cl]2 +/+ redox polymer. Maximum current density achieved in response to 10 mM D-glucose was 18 ± 1 μA cm− 2. Control experiments without redox polymer showed no current response. This demonstrates the efficiency of the osmium-based mediator in electron transfer between the cells having a thick cell wall and the electrode. The findings have significant importance in microbial electrochemistry for further understanding of microbe-electrode interactions.

AB - The Gram-positive bacterium Enterococcus faecalis was investigated to test its ability to electrochemically communicate with electrodes. E. faecalis cells were successfully “wired” to gold electrodes through [Os(2,2′-bipyridine)2-poly(N-vinylimidazole)10Cl]2 +/+ redox polymer. Maximum current density achieved in response to 10 mM D-glucose was 18 ± 1 μA cm− 2. Control experiments without redox polymer showed no current response. This demonstrates the efficiency of the osmium-based mediator in electron transfer between the cells having a thick cell wall and the electrode. The findings have significant importance in microbial electrochemistry for further understanding of microbe-electrode interactions.

KW - Electrochemical communication

KW - Enterococcus faecalis

KW - Gram-positive bacterium

KW - Osmium redox polymer

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U2 - 10.1016/j.elecom.2016.12.010

DO - 10.1016/j.elecom.2016.12.010

M3 - Article

AN - SCOPUS:85008197796

SN - 1388-2481

VL - 75

SP - 56

EP - 59

JO - Electrochemistry Communications

JF - Electrochemistry Communications

ER -

Electrochemical wiring of the Gram-positive bacterium Enterococcus faecalis with osmium redox polymer modified electrodes

Abstract

Subject classification (UKÄ)

Free keywords

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Extracellular electron transfer features of the Gram-positive bacterium Enterococcus faecalis

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