Abstract
The purpose of this review is to draw attention to the use of electrospray ionization mass spectrometry ( ESI-MS) for monitoring the course of enzyme substrate interactions, in the particular case of complex systems in which two substrates participate. The determination and characterization of intra-molecular reactions, especially those that occur in the enzyme active site, is not a trivial task in chemical kinetics, typically requiring long measurement times and relatively expensive techniques such as nuclear magnetic resonance (NMR), X-ray crystallography or electron microscopy ( EM). However, nowadays almost all laboratories are equipped with or else have access to the ESI-MS technique. The aim of this review is to focus on the possibilities of employing even quite simple MS equipment to tackle different applications in studies of complex enzymatic systems.
Original language | English |
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Pages (from-to) | 397-402 |
Journal | Analyst |
Volume | 132 |
Issue number | 5 |
DOIs | |
Publication status | Published - 2007 |
Bibliographical note
The information about affiliations in this record was updated in December 2015.The record was previously connected to the following departments: Biochemistry and Structural Biology (S) (000006142), Analytical Chemistry (S/LTH) (011001004)
Subject classification (UKÄ)
- Biological Sciences
- Analytical Chemistry