Endothelium-derived prostanoids reduce 5-hydroxytryptamine-induced contraction in the human uterine artery

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Abstract

The contribution of endothelium-linked mechanisms to the contraction induced by 5-hydroxytryptamine (5-HT) was investigated in the isolated human uterine artery. 5-HT contracted the uterine artery concentration-dependently. Removal of the endothelium or treatment with the cyclooxygenase inhibitor indomethacin potentiated the contractile response to 5-HT. The nitric oxide synthase inhibitor L-N(G)-monomethyl-arginine (L-NMMA) did not influence the contraction induced by 5-HT. Indomethacin did not affect the response to 5-HT in endothelium-denuded vessels. The 5-HT1 receptor agonist 5-carboxyamidotryptamine (5-CT) did not relax precontracted arteries. Removal of the endothelium did not change the response to 5-HT in the presence of the 5-HT(1B/D) receptor antagonist GR127935 and the 5-HT1A and 5-HT1B receptor antagonist -pindolol. The 5-HT1B receptor antagonist SB224289 did not affect the contraction induced by 5-HT. The results indicate that the 5-HT-induced contraction in the human uterine artery is accompanied by the release of an endothelium-derived relaxing factor (EDRF). This EDRF seems to be a prostanoid, probably prostacyclin (PGI2). The endothelium-linked mechanism seems to be mediated via a 5-HT1 receptor, but it is not possible to further classify the receptor subtype by the information obtained in this study.
Original languageEnglish
Pages (from-to)1947-1951
JournalHuman Reproduction
Volume13
Issue number7
Publication statusPublished - 1998

Bibliographical note

The information about affiliations in this record was updated in December 2015.
The record was previously connected to the following departments: Research Unit for Urogynaecology and Reproductive Pharmacology (013242710), Division of Infection Medicine (BMC) (013024020), Pediatrics/Urology/Gynecology/Endocrinology (013240400)

Subject classification (UKÄ)

  • Obstetrics, Gynecology and Reproductive Medicine
  • Infectious Medicine
  • Clinical Medicine

Free keywords

  • EDRF/endothelium/5-hydroxytryptamine/prostanoids/ uterine artery

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