Engineered baker's yeast as whole-cell biocatalyst for one-pot stereo-selective conversion of amines to alcohols

Research output: Contribution to journalArticlepeer-review

6 Citations (SciVal)

Abstract

Background: One-pot multi-step biocatalysis is advantageous over step-by-step synthesis as it reduces the number of process operation units, leading to significant process intensification. Whole-cell biocatalysis with metabolically active cells is especially valuable since all enzymes can be co-expressed in the cell whose metabolism can be exploited for supply of co-substrates and co-factors. Results: In this study, a heterologous enzymatic system consisting of omega-transaminase and ketone reductase was introduced in Saccharomyces cerevisiae, and evaluated for one-pot stereo-selective conversion of amines to alcohols. The system was applied for simultaneous kinetic resolution of racemic 1-phenylethylamine to (R)-1-phenylethylamine and reduction of the ketone intermediate to (R)-1-phenylethanol. Glucose was used as sole co-substrate for both the supply of amine acceptor and the regeneration of NADPH in the reduction step. Conclusions: The whole-cell biocatalyst was shown to sustain transaminase-reductase-catalyzed enantioselective conversion of amines to alcohols with glucose as co-substrate. The transamination catalyzed by recombinant vanillin aminotransferase from Capsicum chinense proved to be the rate-limiting step as a three-fold increase in transaminase gene copy number led to a two-fold increased conversion. The (R)-selective NADPH-dependent alcohol dehydrogenase from Lactobacillus kefir proved to be efficient in catalyzing the reduction of the acetophenone generated in the transamination reaction.
Original languageEnglish
Article number118
JournalMicrobial Cell Factories
Volume13
DOIs
Publication statusPublished - 2014

Subject classification (UKÄ)

  • Industrial Biotechnology

Keywords

  • Transamination
  • Reduction
  • VAMT
  • Saccharomyces cerevisiae
  • Capsicum
  • chinense
  • Lactobacillus kefir
  • NADPH regeneration
  • Pyruvate
  • PLP
  • Kinetic resolution
  • Asymmetric synthesis

Fingerprint

Dive into the research topics of 'Engineered baker's yeast as whole-cell biocatalyst for one-pot stereo-selective conversion of amines to alcohols'. Together they form a unique fingerprint.

Cite this