Enzymatic oil extraction and positional analysis of omega-3 fatty acids in Nile perch and salmon heads

Betty Mbatia; Dietlind Adlercreutz; Patrick Adlercreutz; Ally Mahadhy; Francis Mulaa; Bo Mattiasson

doi:10.1016/j.procbio.2010.02.010

Enzymatic oil extraction and positional analysis of omega-3 fatty acids in Nile perch and salmon heads

Betty Mbatia, Dietlind Adlercreutz, Patrick Adlercreutz, Ally Mahadhy, Francis Mulaa, Bo Mattiasson

Research output: Contribution to journal › Article › peer-review

Abstract

The use of commercial proteases, bromelain and Protex 30L for oil extraction/recovery of polyunsaturated fatty acids (PUFA) from Nile perch and salmon heads was evaluated. Four phases were obtained after hydrolysis, oily phase, emulsion, aqueous phase and sludge. An increase in water content during the hydrolysis resulted in a decrease in oil yield. Maximum oil yield was obtained when hydrolysis was performed with Protex 30L at 55 C, without pH adjustment or water addition. An oil yield of 11.2% and 15.7% of wet weight was obtained from Nile perch and salmon heads, respectively, compared to 13.8% and 17.6%, respectively obtained using solvent extraction. Fatty acid distribution analysis showed 50% of palmitic acid was in sn-2 position in Nile perch triglycerides (TAG), while only 16% of this fatty acid was in sn-2 position in salmon oil TAG. (C) 2010 Elsevier Ltd. All rights reserved.

Original language	English
Pages (from-to)	815-819
Journal	Process Biochemistry
Volume	45
Issue number	5
DOIs	https://doi.org/10.1016/j.procbio.2010.02.010
Publication status	Published - 2010

Subject classification (UKÄ)

Industrial Biotechnology

Free keywords

DHA
EPA
Positional analysis
Enzymatic extraction
Salmon
Nile perch

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10.1016/j.procbio.2010.02.010

Cite this

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title = "Enzymatic oil extraction and positional analysis of omega-3 fatty acids in Nile perch and salmon heads",

abstract = "The use of commercial proteases, bromelain and Protex 30L for oil extraction/recovery of polyunsaturated fatty acids (PUFA) from Nile perch and salmon heads was evaluated. Four phases were obtained after hydrolysis, oily phase, emulsion, aqueous phase and sludge. An increase in water content during the hydrolysis resulted in a decrease in oil yield. Maximum oil yield was obtained when hydrolysis was performed with Protex 30L at 55 C, without pH adjustment or water addition. An oil yield of 11.2% and 15.7% of wet weight was obtained from Nile perch and salmon heads, respectively, compared to 13.8% and 17.6%, respectively obtained using solvent extraction. Fatty acid distribution analysis showed 50% of palmitic acid was in sn-2 position in Nile perch triglycerides (TAG), while only 16% of this fatty acid was in sn-2 position in salmon oil TAG. (C) 2010 Elsevier Ltd. All rights reserved.",

keywords = "DHA, EPA, Positional analysis, Enzymatic extraction, Salmon, Nile perch",

author = "Betty Mbatia and Dietlind Adlercreutz and Patrick Adlercreutz and Ally Mahadhy and Francis Mulaa and Bo Mattiasson",

year = "2010",

doi = "10.1016/j.procbio.2010.02.010",

language = "English",

volume = "45",

pages = "815--819",

journal = "Process Biochemistry",

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T1 - Enzymatic oil extraction and positional analysis of omega-3 fatty acids in Nile perch and salmon heads

AU - Mbatia, Betty

AU - Adlercreutz, Dietlind

AU - Adlercreutz, Patrick

AU - Mahadhy, Ally

AU - Mulaa, Francis

AU - Mattiasson, Bo

PY - 2010

Y1 - 2010

N2 - The use of commercial proteases, bromelain and Protex 30L for oil extraction/recovery of polyunsaturated fatty acids (PUFA) from Nile perch and salmon heads was evaluated. Four phases were obtained after hydrolysis, oily phase, emulsion, aqueous phase and sludge. An increase in water content during the hydrolysis resulted in a decrease in oil yield. Maximum oil yield was obtained when hydrolysis was performed with Protex 30L at 55 C, without pH adjustment or water addition. An oil yield of 11.2% and 15.7% of wet weight was obtained from Nile perch and salmon heads, respectively, compared to 13.8% and 17.6%, respectively obtained using solvent extraction. Fatty acid distribution analysis showed 50% of palmitic acid was in sn-2 position in Nile perch triglycerides (TAG), while only 16% of this fatty acid was in sn-2 position in salmon oil TAG. (C) 2010 Elsevier Ltd. All rights reserved.

AB - The use of commercial proteases, bromelain and Protex 30L for oil extraction/recovery of polyunsaturated fatty acids (PUFA) from Nile perch and salmon heads was evaluated. Four phases were obtained after hydrolysis, oily phase, emulsion, aqueous phase and sludge. An increase in water content during the hydrolysis resulted in a decrease in oil yield. Maximum oil yield was obtained when hydrolysis was performed with Protex 30L at 55 C, without pH adjustment or water addition. An oil yield of 11.2% and 15.7% of wet weight was obtained from Nile perch and salmon heads, respectively, compared to 13.8% and 17.6%, respectively obtained using solvent extraction. Fatty acid distribution analysis showed 50% of palmitic acid was in sn-2 position in Nile perch triglycerides (TAG), while only 16% of this fatty acid was in sn-2 position in salmon oil TAG. (C) 2010 Elsevier Ltd. All rights reserved.

KW - DHA

KW - EPA

KW - Positional analysis

KW - Enzymatic extraction

KW - Salmon

KW - Nile perch

U2 - 10.1016/j.procbio.2010.02.010

DO - 10.1016/j.procbio.2010.02.010

M3 - Article

SN - 1873-3298

VL - 45

SP - 815

EP - 819

JO - Process Biochemistry

JF - Process Biochemistry

IS - 5

ER -

Enzymatic oil extraction and positional analysis of omega-3 fatty acids in Nile perch and salmon heads

Abstract

Subject classification (UKÄ)

Free keywords

Access to Document

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Cite this