Ethanol stimulates basal and serotonin-induced formation of [32P]phosphatidic acid in human platelets

Per Simonsson; Istvan Ferencz; Christer Alling

doi:10.1016/0376-8716(89)90081-1

Ethanol stimulates basal and serotonin-induced formation of [32P]phosphatidic acid in human platelets

Per Simonsson, Istvan Ferencz, Christer Alling

Research output: Contribution to journal › Article › peer-review

Abstract

The addition of serotonin to preparations of 32P-labelled human platelets resulted in a time- and dose-dependent hydrolysis of [32P]phosphatidylinositol 4,5-bisphosphate (PIP2) and formation of [32P]phosphatidic acid (PA). This response was inhibited by the serotonin2 receptor antagonist ritanserin, indicating that the stimulation was mediated via the serotonin2 receptor. The addition of 50-150 mM of ethanol prior to stimulation with 10(-5) M serotonin resulted in an increased accumulation of [32P]PA, but had no effect on [32P]PIP2. Ethanol stimulated [32P]PA formation at all serotonin concentrations studied (10(-7)-10(-5) M). Furthermore, in the absence of serotonin, ethanol increased basal [32P]PA formation.

Original language	English
Pages (from-to)	169-174
Journal	Drug and Alcohol Dependence
Volume	24
Issue number	2
DOIs	https://doi.org/10.1016/0376-8716(89)90081-1
Publication status	Published - 1989

Subject classification (UKÄ)

Drug Abuse and Addiction

Free keywords

ethanol
phospholipase C
serotonin
phosphatidic acid
platelets

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/0376-8716(89)90081-1

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title = "Ethanol stimulates basal and serotonin-induced formation of [32P]phosphatidic acid in human platelets",

abstract = "The addition of serotonin to preparations of 32P-labelled human platelets resulted in a time- and dose-dependent hydrolysis of [32P]phosphatidylinositol 4,5-bisphosphate (PIP2) and formation of [32P]phosphatidic acid (PA). This response was inhibited by the serotonin2 receptor antagonist ritanserin, indicating that the stimulation was mediated via the serotonin2 receptor. The addition of 50-150 mM of ethanol prior to stimulation with 10(-5) M serotonin resulted in an increased accumulation of [32P]PA, but had no effect on [32P]PIP2. Ethanol stimulated [32P]PA formation at all serotonin concentrations studied (10(-7)-10(-5) M). Furthermore, in the absence of serotonin, ethanol increased basal [32P]PA formation.",

keywords = "ethanol, phospholipase C, serotonin, phosphatidic acid, platelets",

author = "Per Simonsson and Istvan Ferencz and Christer Alling",

year = "1989",

doi = "10.1016/0376-8716(89)90081-1",

language = "English",

volume = "24",

pages = "169--174",

journal = "Drug and Alcohol Dependence",

issn = "1879-0046",

publisher = "Elsevier",

number = "2",

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TY - JOUR

T1 - Ethanol stimulates basal and serotonin-induced formation of [32P]phosphatidic acid in human platelets

AU - Simonsson, Per

AU - Ferencz, Istvan

AU - Alling, Christer

PY - 1989

Y1 - 1989

N2 - The addition of serotonin to preparations of 32P-labelled human platelets resulted in a time- and dose-dependent hydrolysis of [32P]phosphatidylinositol 4,5-bisphosphate (PIP2) and formation of [32P]phosphatidic acid (PA). This response was inhibited by the serotonin2 receptor antagonist ritanserin, indicating that the stimulation was mediated via the serotonin2 receptor. The addition of 50-150 mM of ethanol prior to stimulation with 10(-5) M serotonin resulted in an increased accumulation of [32P]PA, but had no effect on [32P]PIP2. Ethanol stimulated [32P]PA formation at all serotonin concentrations studied (10(-7)-10(-5) M). Furthermore, in the absence of serotonin, ethanol increased basal [32P]PA formation.

AB - The addition of serotonin to preparations of 32P-labelled human platelets resulted in a time- and dose-dependent hydrolysis of [32P]phosphatidylinositol 4,5-bisphosphate (PIP2) and formation of [32P]phosphatidic acid (PA). This response was inhibited by the serotonin2 receptor antagonist ritanserin, indicating that the stimulation was mediated via the serotonin2 receptor. The addition of 50-150 mM of ethanol prior to stimulation with 10(-5) M serotonin resulted in an increased accumulation of [32P]PA, but had no effect on [32P]PIP2. Ethanol stimulated [32P]PA formation at all serotonin concentrations studied (10(-7)-10(-5) M). Furthermore, in the absence of serotonin, ethanol increased basal [32P]PA formation.

KW - ethanol

KW - phospholipase C

KW - serotonin

KW - phosphatidic acid

KW - platelets

U2 - 10.1016/0376-8716(89)90081-1

DO - 10.1016/0376-8716(89)90081-1

M3 - Article

SN - 1879-0046

VL - 24

SP - 169

EP - 174

JO - Drug and Alcohol Dependence

JF - Drug and Alcohol Dependence

IS - 2

ER -

Ethanol stimulates basal and serotonin-induced formation of [32P]phosphatidic acid in human platelets

Abstract

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