Ethanolic fermentation of xylose with Saccharomyces cerevisiae harboring the Thermus thermophilus xylA gene, which expresses an active xylose (glucose) isomerase

M Walfridsson; X Bao; M Anderlund; G Lilius; L Bülow; B Hahn-Hägerdal

Ethanolic fermentation of xylose with Saccharomyces cerevisiae harboring the Thermus thermophilus xylA gene, which expresses an active xylose (glucose) isomerase

M Walfridsson, X Bao, M Anderlund, G Lilius, L Bülow, B Hahn-Hägerdal

Pure and Applied Biochemistry

Research output: Contribution to journal › Article › peer-review

Abstract

The Thermus thermophilus xylA gene encoding xylose (glucose) isomerase was cloned and expressed in Saccharomyces cerevisiae under the control of the yeast PGK1 promoter. The recombinant xylose isomerase showed the highest activity at 85 degrees C with a specific activity of 1.0 U mg-1. A new functional metabolic pathway in S. cerevisiae with ethanol formation during oxygen-limited xylose fermentation was demonstrated. Xylitol and acetic acid were also formed during the fermentation.

Original language	English
Pages (from-to)	4648-51
Number of pages	4
Journal	Applied and Environmental Microbiology
Volume	62
Issue number	12
Publication status	Published - 1996 Dec

Subject classification (UKÄ)

Microbiology

Free keywords

Aldose-Ketose Isomerases
Base Sequence
Carbohydrate Epimerases
Ethanol
Fermentation
Genes, Bacterial
Molecular Sequence Data
Phosphoglycerate Kinase
Recombinant Proteins
Saccharomyces cerevisiae
Thermus thermophilus
Xylose

Access to Document

http://aem.asm.org/content/62/12/4648.abstract

Cite this

@article{7abdaa5bde4742d6b0331a5e1f263eb0,

title = "Ethanolic fermentation of xylose with Saccharomyces cerevisiae harboring the Thermus thermophilus xylA gene, which expresses an active xylose (glucose) isomerase",

abstract = "The Thermus thermophilus xylA gene encoding xylose (glucose) isomerase was cloned and expressed in Saccharomyces cerevisiae under the control of the yeast PGK1 promoter. The recombinant xylose isomerase showed the highest activity at 85 degrees C with a specific activity of 1.0 U mg-1. A new functional metabolic pathway in S. cerevisiae with ethanol formation during oxygen-limited xylose fermentation was demonstrated. Xylitol and acetic acid were also formed during the fermentation.",

keywords = "Aldose-Ketose Isomerases, Base Sequence, Carbohydrate Epimerases, Ethanol, Fermentation, Genes, Bacterial, Molecular Sequence Data, Phosphoglycerate Kinase, Recombinant Proteins, Saccharomyces cerevisiae, Thermus thermophilus, Xylose",

author = "M Walfridsson and X Bao and M Anderlund and G Lilius and L B{\"u}low and B Hahn-H{\"a}gerdal",

year = "1996",

month = dec,

language = "English",

volume = "62",

pages = "4648--51",

journal = "Applied and Environmental Microbiology",

issn = "0099-2240",

publisher = "American Society for Microbiology",

number = "12",

}

TY - JOUR

T1 - Ethanolic fermentation of xylose with Saccharomyces cerevisiae harboring the Thermus thermophilus xylA gene, which expresses an active xylose (glucose) isomerase

AU - Walfridsson, M

AU - Bao, X

AU - Anderlund, M

AU - Lilius, G

AU - Bülow, L

AU - Hahn-Hägerdal, B

PY - 1996/12

Y1 - 1996/12

N2 - The Thermus thermophilus xylA gene encoding xylose (glucose) isomerase was cloned and expressed in Saccharomyces cerevisiae under the control of the yeast PGK1 promoter. The recombinant xylose isomerase showed the highest activity at 85 degrees C with a specific activity of 1.0 U mg-1. A new functional metabolic pathway in S. cerevisiae with ethanol formation during oxygen-limited xylose fermentation was demonstrated. Xylitol and acetic acid were also formed during the fermentation.

AB - The Thermus thermophilus xylA gene encoding xylose (glucose) isomerase was cloned and expressed in Saccharomyces cerevisiae under the control of the yeast PGK1 promoter. The recombinant xylose isomerase showed the highest activity at 85 degrees C with a specific activity of 1.0 U mg-1. A new functional metabolic pathway in S. cerevisiae with ethanol formation during oxygen-limited xylose fermentation was demonstrated. Xylitol and acetic acid were also formed during the fermentation.

KW - Aldose-Ketose Isomerases

KW - Base Sequence

KW - Carbohydrate Epimerases

KW - Ethanol

KW - Fermentation

KW - Genes, Bacterial

KW - Molecular Sequence Data

KW - Phosphoglycerate Kinase

KW - Recombinant Proteins

KW - Saccharomyces cerevisiae

KW - Thermus thermophilus

KW - Xylose

M3 - Article

C2 - 8953736

SN - 0099-2240

VL - 62

SP - 4648

EP - 4651

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

IS - 12

ER -