Evaluation of electron mediators for the electromicrobial reduction of chloropyruvate by Proteus vulgaris cells

Mats Andersson, Hans Holmberg, Patrick Adlercreutz

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Different electron mediators were evaluated for the electromicrobial reduction of chloropyruvate to chlorolactate. The reaction was catalyzed by a mediatol-dependent D-lactate dehydrogenase in whole cells of Proteus vulgaris and yielded D-(S)-chlorolactate with high optical purity (> 97% e.e.). The ideal mediatol should favor a high rate in the enzymatic reaction and a low rate in the nonenzymatic dehalogenation of the substrate. The latter reaction causes the formation of pyruvate and subsequently lactate. Viologens as mediators provided better yields than cobalt sepulchrate, safranine O, and an anthraquinone derivative. The best results were obtained with 1,1'-carbamoylmethylviologen as mediator.

    Original languageEnglish
    Pages (from-to)150-156
    Number of pages7
    JournalEnzyme and Microbial Technology
    Volume20
    Issue number2
    DOIs
    Publication statusPublished - 1997 Feb 1

    Subject classification (UKÄ)

    • Biocatalysis and Enzyme Technology

    Free keywords

    • Artificial electron mediators
    • D-chlorolactic acid
    • Electromicrobial reduction
    • Proteus vulgaris

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