Abstract
Different electron mediators were evaluated for the electromicrobial reduction of chloropyruvate to chlorolactate. The reaction was catalyzed by a mediatol-dependent D-lactate dehydrogenase in whole cells of Proteus vulgaris and yielded D-(S)-chlorolactate with high optical purity (> 97% e.e.). The ideal mediatol should favor a high rate in the enzymatic reaction and a low rate in the nonenzymatic dehalogenation of the substrate. The latter reaction causes the formation of pyruvate and subsequently lactate. Viologens as mediators provided better yields than cobalt sepulchrate, safranine O, and an anthraquinone derivative. The best results were obtained with 1,1'-carbamoylmethylviologen as mediator.
Original language | English |
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Pages (from-to) | 150-156 |
Number of pages | 7 |
Journal | Enzyme and Microbial Technology |
Volume | 20 |
Issue number | 2 |
DOIs | |
Publication status | Published - 1997 Feb 1 |
Subject classification (UKÄ)
- Biocatalysis and Enzyme Technology
Free keywords
- Artificial electron mediators
- D-chlorolactic acid
- Electromicrobial reduction
- Proteus vulgaris