Evaluation of ethanol effects on PLC signal transduction pathways using cell lines of neuronal origin

Christer Alling, O Bergman, Christer Larsson, Per Simonsson

Research output: Contribution to journalArticlepeer-review

Abstract

Human neuroblastoma cells SH-SY5Y and neuroblastoma-glioma cells NG 108-15 have been used as models for the elucidation of the effects of ethanol on receptor-mediated phospholipase C activity, c-fos mRNA expression and protein kinase C activity. Cells were exposed to ethanol (0-200 mM) for varying periods up to seven days. Agonist stimulated events were obtained in NG 108-15 cells with bradykinin and in SH-SY5Y cells with carbachol. Chronic ethanol exposure reduced the agonist-stimulated formation of inositol 1,4,5-trisphosphate in NG 108-15 cells and in SH-SY5Y cells. 100 mM ethanol for seven days increased the membrane bound and cytosolic forms of protein kinase C activity in SH-SY5Y cells. Carbachol (1 mM) induced a maximal c-fos mRNA response after 40 minutes in SH-SY5Y cells, an effect that could be mimicked through protein kinase C stimulation by phorbol esters.
Original languageEnglish
Pages (from-to)295-299
JournalAlcohol and alcoholism. Supplement
Volume2
Publication statusPublished - 1993

Bibliographical note

The information about affiliations in this record was updated in December 2015.
The record was previously connected to the following departments: Division of Clinical Chemistry and Pharmacology (013250300), Tumour Cell Biology (013017530), Clinical Chemistry, Malmö (013016000)

Subject classification (UKÄ)

  • Medicinal Chemistry

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