Abstract
Human neuroblastoma cells SH-SY5Y and neuroblastoma-glioma cells NG 108-15 have been used as models for the elucidation of the effects of ethanol on receptor-mediated phospholipase C activity, c-fos mRNA expression and protein kinase C activity. Cells were exposed to ethanol (0-200 mM) for varying periods up to seven days. Agonist stimulated events were obtained in NG 108-15 cells with bradykinin and in SH-SY5Y cells with carbachol. Chronic ethanol exposure reduced the agonist-stimulated formation of inositol 1,4,5-trisphosphate in NG 108-15 cells and in SH-SY5Y cells. 100 mM ethanol for seven days increased the membrane bound and cytosolic forms of protein kinase C activity in SH-SY5Y cells. Carbachol (1 mM) induced a maximal c-fos mRNA response after 40 minutes in SH-SY5Y cells, an effect that could be mimicked through protein kinase C stimulation by phorbol esters.
| Original language | English |
|---|---|
| Pages (from-to) | 295-299 |
| Journal | Alcohol and alcoholism. Supplement |
| Volume | 2 |
| Publication status | Published - 1993 |
Bibliographical note
The information about affiliations in this record was updated in December 2015.The record was previously connected to the following departments: Division of Clinical Chemistry and Pharmacology (013250300), Tumour Cell Biology (013017530), Clinical Chemistry, Malmö (013016000)
Subject classification (UKÄ)
- Medicinal Chemistry